Rabbit Rak Polyclonal Antibody | anti-Rak antibody

Rak (N103) Polyclonal Antibody

Cat. Num. AAA3002013

• Gene Names: FRK; GTK; RAK; PTK5
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: Rak; Polyclonal Antibody; Rak (N103) Polyclonal Antibody; Tyrosine-protein kinase FRK; FYN-related kinase; Nuclear tyrosine protein kinase RAK; Protein-tyrosine kinase 5; PTK5; RAK; FRK; anti-Rak antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Rak (N103) polyclonal antibody detects endogenous levels of Rak protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 505

• Applicable Applications for anti-Rak antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: WB: 1:500-1:1000; IHC: 1:50-1:200; IF: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids 80-120 of Human Rak.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of Rak (N103) pAb at 1:500 dilutionLane1:L02 whole cell lysate (40ug) Lane2:HEK293T whole cell lysate (40ug) Lane3:CT26 whole cell lysate (40ug) Lane4:C6 whole cell lysate (40ug))

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of Rak (N103) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.)

Western Blot (WB)

(Western Blot (WB) analysis of Rak (N103) pAb at 1:500 dilutionLane1:HEK293T whole cell lysate (40ug) Lane2:L02 whole cell lysate (40ug) Lane3:786-O whole cell lysate (40ug))

Related Product Information for anti-Rak antibody

Src is the human homolog of the v-src gene of the Rous sarcoma virus, also known as avian sarcoma virus or ASV. Src is the first proto-oncogenic nonreceptor tyrosine kinase characterized in human. By virtue of common structural motifs, the Src family is composed of nine members in vertebrates, including Src, Yes, Fgr, Frk, Fyn, Lyn, Hck, Lck and Blk. Src-family kinases transduce signals that are involved in the control of a variety of cellular processes, including proliferation, differentiation, motility, and adhesion. Src-family kinases contain an amino terminal cell membrane anchor followed by an SH3 domain and an SH2 domain involved in modular association and activation, respectively. Frk (Rak) is an epithelial tissue-specific kinase. The human Rak gene maps to chromosome 6q21-q22.3 and encodes a 505 amino acid protein.

NCBI and Uniprot Product Information

• NCBI GI #: 4503787
• NCBI GeneID: 2444
• NCBI Accession #: NP_002022.1
• NCBI GenBank Nucleotide #: NP_002022.1
• UniProt Accession #: P42685
• Molecular Weight: ~ 55kDa
• NCBI Official Full Name: tyrosine-protein kinase FRK
• NCBI Official Synonym Full Names: fyn related Src family tyrosine kinase
• NCBI Official Symbol: FRK
• NCBI Official Synonym Symbols: GTK; RAK; PTK5
• NCBI Protein Information: tyrosine-protein kinase FRK
• UniProt Protein Name: Tyrosine-protein kinase FRK
• UniProt Gene Name: FRK
• UniProt Synonym Gene Names: PTK5; RAK
• UniProt Entry Name: FRK_HUMAN

NCBI Description

The protein encoded by this gene belongs to the TYR family of protein kinases. This tyrosine kinase is a nuclear protein and may function during G1 and S phase of the cell cycle and suppress growth. [provided by RefSeq, Jul 2008]

Uniprot Description

FRK: a tyrosine kinase of the Src family. Expressed in cell lines derived from tissues of lymphoid, brain, breast, colon and bladder origin. A nuclear protein that may function during G1 and S phase of the cell cycle to suppress growth.

Protein type: Protein kinase, TK; EC 2.7.10.2; Tumor suppressor; Kinase, protein; Protein kinase, tyrosine (non-receptor); TK group; Src family

Chromosomal Location of Human Ortholog: 6q21-q22.3

Cellular Component: extrinsic to internal side of plasma membrane; cytoplasm; intracellular; nucleus

Molecular Function: protein binding; non-membrane spanning protein tyrosine kinase activity; ATP binding; receptor binding

Biological Process: negative regulation of cell proliferation; cell migration; innate immune response; cell differentiation; actin cytoskeleton organization and biogenesis; protein amino acid phosphorylation; transmembrane receptor protein tyrosine kinase signaling pathway; regulation of cell proliferation