Rabbit UCN Polyclonal Antibody | anti-UCN antibody

UCN (A103) Polyclonal Antibody

Cat. Num. AAA3001171

• Gene Names: UCN; UI; UROC
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: UCN; Polyclonal Antibody; UCN (A103) Polyclonal Antibody; Urocortin; anti-UCN antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Urocortin (A103) polyclonal antibody detects endogenous levels of Urocortin protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 124

• Applicable Applications for anti-UCN antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:1000; IHC: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids 80-130 of Human UCN.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of UCN (A103) polyclonal antibody at 1:500 dilutionLane1:HEK293T whole cell lysateLane2:sp2/0 whole cell lysateLane3:PC12 whole cell lysate)

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of UCN (A103) pAb in paraffin-embedded human colorectal carcinoma tissue at 1:100.)

Related Product Information for anti-UCN antibody

Urocortins 1, 2 and 3 are the mammalian homologs of fish urotensin I. Mammalian urocortin is a member of the corticotropin-releasing hormone(CRH) family that is expressed in a region of the rat midbrain. The distinct expression pattern in discrete brain regions suggests that it influences such behavior as feeding, anxiety and auditory processing. Synthetic human urocortin binds with high affinity to CRH receptors and acts in vitro to release ACTH from dispersed rat anterior pituitary cells. It is suggested that urocortin may be an endogenous CRF-like factor in the brain responsible for the effects of stress on appetite. The gene which encodes urocortin maps to human chromosome 2. Specifically, urocortin III is a specific ligand for CRF type 2 receptor which mediates stress-coping responses. Human urocortin I and III are expressed in the human heart. Urocortin III is also expressed in the pituitary gland, adrenal gland, GI tract, ovary, spleen, brain and kidney.

NCBI and Uniprot Product Information

• NCBI GI #: 4507803
• NCBI GeneID: 7349
• NCBI Accession #: NP_003344.1
• NCBI GenBank Nucleotide #: NP_003344.1
• UniProt Accession #: P55089
• Molecular Weight: ~ 13kDa
• NCBI Official Full Name: urocortin preproprotein
• NCBI Official Synonym Full Names: urocortin
• NCBI Official Symbol: UCN
• NCBI Official Synonym Symbols: UI; UROC
• NCBI Protein Information: urocortin
• UniProt Protein Name: Urocortin
• Protein Family: Urocortin
• UniProt Gene Name: UCN
• UniProt Entry Name: UCN1_HUMAN

NCBI Description

This gene encodes a member of the sauvagine/corticotropin-releasing factor/urotensin I family. The encoded preproprotein is proteolytically processed to generate the mature peptide, an endogenous ligand for both corticotropin-releasing factor receptor 1 and corticotropin-releasing factor receptor 2. In the brain this peptide may be responsible for the effects of stress on appetite. This peptide may also play a role in mood disorders, neurodegeneration, and skeletal system disorders. In spite of the gene family name similarity, the product of this gene has no sequence similarity to urotensin-2. [provided by RefSeq, Feb 2016]

Uniprot Description

UCN: Acts in vitro to stimulate the secretion of adrenocorticotropic hormone (ACTH). Binds with high affinity to CRF receptor types 1, 2-alpha, and 2-beta. Belongs to the sauvagine/corticotropin-releasing factor/urotensin I family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 2p23.3

Cellular Component: dendrite; extracellular region; perikaryon; nerve terminal

Molecular Function: corticotropin-releasing hormone receptor 1 binding; neuropeptide hormone activity; histone deacetylase inhibitor activity; protein binding; corticotropin-releasing hormone receptor 2 binding

Biological Process: regulation of synaptic transmission, glutamatergic; positive regulation of translation; negative regulation of hormone secretion; drinking behavior; response to glucocorticoid stimulus; positive regulation of collagen biosynthetic process; response to pain; negative regulation of cell size; female pregnancy; pancreatic juice secretion; positive regulation of vasodilation; negative regulation of appetite; response to estradiol stimulus; positive regulation of adrenocorticotropic hormone secretion; sensory perception of sound; neuropeptide signaling pathway; positive regulation of cAMP biosynthetic process; negative regulation of blood pressure; inflammatory response; associative learning; neurite development; negative regulation of histone deacetylation; activation of protein kinase A; positive regulation of interleukin-6 production; social behavior; positive regulation of cell growth; positive regulation of peptidyl-serine phosphorylation; positive regulation of vascular permeability; G-protein coupled receptor protein signaling pathway; aerobic respiration; response to oxidative stress; positive regulation of DNA replication; negative regulation of apoptosis