Rabbit Amylin Polyclonal Antibody | anti-H28 antibody

Amylin (H28) Polyclonal Antibody

Cat. Num. AAA3000970

• Gene Names: IAPP; DAP; IAP
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry, Immunofluorescence
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: Amylin; Polyclonal Antibody; Amylin (H28) Polyclonal Antibody; Islet amyloid polypeptide; Diabetes-associated peptide; DAP; Insulinoma amyloid peptide; IAPP; anti-H28 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Amylin (H28) polyclonal antibody detects endogenous levels of Amylin protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 89

• Applicable Applications for anti-H28 antibody: Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: IHC: 1:50-1:200; IF: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids 1-50 of Human Amylin.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of Amylin (H28) pAb in paraffin-embedded mouse brain tissue at 1:50,showing distinct cytoplasmic membrane and secreted staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.)

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of Amylin (H28) pAb in paraffin-embedded human colon carcinoma tissue at 1:50,showing distinct cytoplasmic membrane and secreted staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.)

Related Product Information for anti-H28 antibody

Adrenomedullin (AM), alpha- and beta-calcitonin gene-related peptide(CGRP), calcitonin (CT), and amylin are homologous polypeptides with overlapping biological actions such as vasodilatation and inhibition of bone resorption. Amylin (Islet/insulinoma amyloid polypeptide or IAPP) is a 37-amino acid monomeric polypeptide isolated from pancreatic amyloid. This protein, with a calculated molecular mass of 4 kDa, is a major component of amyloid-rich pancreatic extracts of three type 2 diabetic patients. IAPP has cysteine residues in positions 2 and 7, a feature found in all known calcitonin gene-related peptides. IAPP shows 46% amino acid sequence homology with CGRP II. Since IAPP has been demonstrated immunochemically in normal beta cells of several mammals, it probably has an important role in respect to pancreatic islet function.

NCBI and Uniprot Product Information

• NCBI GI #: 4557655
• NCBI GeneID: 3375
• NCBI Accession #: NP_000406.1
• NCBI GenBank Nucleotide #: NP_000406.1
• UniProt Accession #: P10997
• Molecular Weight: ~ 10kDa
• NCBI Official Full Name: islet amyloid polypeptide preproprotein
• NCBI Official Synonym Full Names: islet amyloid polypeptide
• NCBI Official Symbol: IAPP
• NCBI Official Synonym Symbols: DAP; IAP
• NCBI Protein Information: islet amyloid polypeptide
• UniProt Protein Name: Islet amyloid polypeptide
• UniProt Gene Name: IAPP
• UniProt Synonym Gene Names: DAP
• UniProt Entry Name: IAPP_HUMAN

NCBI Description

This gene encodes a member of the calcitonin family of peptide hormones. This hormone is released from pancreatic beta cells following food intake to regulate blood glucose levels and act as a satiation signal. Human patients with type 1 and advanced type 2 diabetes exhibit reduced levels of the encoded hormone in blood and pancreas. This protein also exhibits a bactericidal, antimicrobial activity. [provided by RefSeq, Jul 2016]

Uniprot Description

IAPP: Selectively inhibits insulin-stimulated glucose utilization and glycogen deposition in muscle, while not affecting adipocyte glucose metabolism. Belongs to the calcitonin family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 12p12.1

Cellular Component: extracellular space; cell soma; extracellular region

Molecular Function: identical protein binding; hormone activity; receptor binding

Biological Process: negative regulation of cell differentiation; cell-cell signaling; apoptosis; eating behavior; signal transduction; sensory perception of pain; negative regulation of bone resorption; endocrine pancreas development