UBE2B / Ubiquitin-Conjugating Enzyme E2 B Recombinant Protein | UBE2B recombinant protein

Human UBE2B / Ubiquitin-Conjugating Enzyme E2 B Recombinant Protein

• Gene Names: UBE2B; HR6B; UBC2; HHR6B; RAD6B; E2-17kDa
• Applications: ELISA, Western Blot
• Purity: >95% by SDS-PAGE
• Synonyms: UBE2B / Ubiquitin-Conjugating Enzyme E2 B; Human UBE2B / Ubiquitin-Conjugating Enzyme E2 B Recombinant Protein; Ubiquitin-conjugating enzyme E2 B; E2 ubiquitin-conjugating enzyme B; RAD6 homolog B; HR6B; Ubiquitin carrier protein B; Ubiquitin-conjugating enzyme E2-17 kDa; Ubiquitin-protein ligase B; RAD6B; UBE2B recombinant protein

• Host: E Coli
• Purity/Purification: >95% by SDS-PAGE
• Form/Format: Liquid; 50mM NaH2PO4, 500mM NaCl Buffer with 500mM Imidazole, 10% Glycerol (PH8.0)
• Concentration: Reconstitution Dependent (varies by lot)
• Sequence Length: 152

• Applicable Applications for UBE2B recombinant protein: ELISA (EIA), Western Blot (WB), Antigen Protein (AP), Protein Array
• Species: Human
• Tag: His
• Subunit: Interacts with RAD18, UBR2 and WAC.
• Entry Function: Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In association with the E3 enzyme BRE1 (RNF20 and/or RNF40), it plays a role in transcription regulation by catalyzing the monoubiquitination of histone H2B at 'Lys-120' to form H2BK120ub1. H2BK120ub1 gives a specific tag for epigenetic transcriptional activation, elongation by RNA polymerase II, telomeric silencing, and is also a prerequisite for H3K4me and H3K79me formation. In vitro catalyzes 'Lys-11'-, as well as 'Lys-48'- and 'Lys-63'-linked polyubiquitination. Required for postreplication repair of UV-damaged DNA. Associates to the E3 ligase RAD18 to form the UBE2B-RAD18 ubiquitin ligase complex involved in mono-ubiquitination of DNA-associated PCNA on 'Lys-164'. May be involved in neurite outgrowth.
• Preparation and Storage: Store at -20 degree C. Avoid repeated freezing and thawing.

NCBI and Uniprot Product Information

• NCBI GI #: 52783814
• NCBI GeneID: 7320
• UniProt Accession #: P63146
• NCBI Official Full Name: Ubiquitin-conjugating enzyme E2 B
• NCBI Official Synonym Full Names: ubiquitin conjugating enzyme E2 B
• NCBI Official Symbol: UBE2B
• NCBI Official Synonym Symbols: HR6B; UBC2; HHR6B; RAD6B; E2-17kDa
• NCBI Protein Information: ubiquitin-conjugating enzyme E2 B
• UniProt Protein Name: Ubiquitin-conjugating enzyme E2 B
• Protein Family: Ubiquitin-conjugating enzyme
• UniProt Gene Name: UBE2B
• UniProt Synonym Gene Names: RAD6B; HR6B; hHR6B
• UniProt Entry Name: UBE2B_HUMAN

NCBI Description

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is required for post-replicative DNA damage repair. Its protein sequence is 100% identical to the mouse, rat, and rabbit homologs, which indicates that this enzyme is highly conserved in eukaryotic evolution. [provided by RefSeq, Jul 2008]

Uniprot Description

UBE2B: a ubiquitin-protein ligase, a member of the ubiquitin-conjugating enzyme family. Homologous to the yeast DNA repair gene RAD6. Interacts with RAD18. Required for postreplication repair of UV-damaged DNA.

Protein type: Ligase; EC 6.3.2.19; Ubiquitin conjugating system; Ubiquitin ligase

Chromosomal Location of Human Ortholog: 5q31.1

Cellular Component: XY body; nuclear chromatin; cytoplasm; plasma membrane; replication fork; chromatin; nucleus

Molecular Function: protein binding; small conjugating protein ligase activity; ubiquitin protein ligase binding; ubiquitin-protein ligase activity; ATP binding; ligase activity

Biological Process: response to drug; protein monoubiquitination; ubiquitin-dependent protein catabolic process; proteasomal ubiquitin-dependent protein catabolic process; protein autoubiquitination; protein polyubiquitination; protein stabilization; in utero embryonic development; postreplication repair; protein ubiquitination; Wnt receptor signaling pathway through beta-catenin; DNA repair; negative regulation of histone phosphorylation; cellular response to insulin stimulus; maintenance of chromatin silencing; response to hypoxia; sperm axoneme assembly; spermatogenesis; chiasma formation; response to DNA damage stimulus; histone H2A ubiquitination; response to UV; negative regulation of apoptosis