IFITM3 / Interferon-Induced Transmembrane Protein 3 Recombinant Protein | IFITM3 recombinant protein

Human IFITM3 / Interferon-Induced Transmembrane Protein 3 Recombinant Protein

• Gene Names: IFITM3; 1-8U; IP15; DSPA2b
• Applications: ELISA, Western Blot
• Purity: >95% by SDS-PAGE
• Synonyms: IFITM3 / Interferon-Induced Transmembrane Protein 3; Human IFITM3 / Interferon-Induced Transmembrane Protein 3 Recombinant Protein; Interferon-induced transmembrane protein 3; Dispanin subfamily A member 2b; DSPA2b; Interferon-inducible protein 1-8U; IFITM3 recombinant protein

• Host: E Coli
• Purity/Purification: >95% by SDS-PAGE
• Form/Format: Liquid; 50mM NaH2PO4, 500mM NaCl Buffer with 500mM Imidazole, 10% Glycerol (PH8.0)
• Concentration: Reconstitution Dependent (varies by lot)
• Sequence Length: 133

• Applicable Applications for IFITM3 recombinant protein: ELISA (EIA), Western Blot (WB), Antigen Protein (AP), Protein Array
• Species: Human
• Tag: His
• Subunit: Interacts with ATP6V0B and CD81 (By similarity). Interacts with SPP1; the interaction reduces OPN expression. Interacts with VAPA.
• Entry Function: IFN-induced antiviral protein which disrupts intracellular cholesterol homeostasis. Inhibits the entry of viruses to the host cell cytoplasm by preventing viral fusion with cholesterol depleted endosomes. May inactivate new enveloped viruses which buds out of the infected cell, by letting them go out with a cholesterol depleted membrane. Active against multiple viruses, including influenza A virus, SARS coronavirus (SARS-CoV), Marburg virus (MARV) and Ebola virus (EBOV), Dengue virus (DNV), West Nile virus (WNV), human immunodeficiency virus type 1 (HIV-1) and vesicular stomatitis virus (VSV). Can inhibit: influenza virus hemagglutinin protein-mediated viral entry, MARV and EBOV GP1,2-mediated viral entry, SARS-CoV S protein-mediated viral entry and VSV G protein-mediated viral entry. Plays a critical role in the structural stability and function of vacuolar ATPase (v-ATPase). Establishes physical contact with the v-ATPase of endosomes which is critical for proper clathrin localization and is also required for the function of the v-ATPase to lower the pH in phagocytic endosomes thus establishing an antiviral state.
• Preparation and Storage: Store at -20 degree C. Avoid repeated freezing and thawing.

NCBI and Uniprot Product Information

• NCBI GI #: 20178301
• NCBI GeneID: 10410
• UniProt Accession #: Q01628
• NCBI Official Full Name: Interferon-induced transmembrane protein 3
• NCBI Official Synonym Full Names: interferon induced transmembrane protein 3
• NCBI Official Symbol: IFITM3
• NCBI Official Synonym Symbols: 1-8U; IP15; DSPA2b
• NCBI Protein Information: interferon-induced transmembrane protein 3
• UniProt Protein Name: Interferon-induced transmembrane protein 3
• Protein Family: Interferon-induced transmembrane protein
• UniProt Gene Name: IFITM3
• UniProt Synonym Gene Names: DSPA2b
• UniProt Entry Name: IFM3_HUMAN

NCBI Description

The protein encoded by this gene is an interferon-induced membrane protein that helps confer immunity to influenza A H1N1 virus, West Nile virus, and dengue virus. Two transcript variants, only one of them protein-coding, have been found for this gene. Another variant encoding an N-terminally truncated isoform has been reported, but the full-length nature of this variant has not been determined. [provided by RefSeq, May 2012]

Uniprot Description

IFITM3: IFN-induced antiviral protein that mediates cellular innate immunity to at least three major human pathogens, namely influenza A H1N1 virus, West Nile virus (WNV), and dengue virus (WNV), by inhibiting the early step(s) of replication. Interacts with SPP1; the interaction reduces OPN expression. By IFN-alpha and IFNG/IFN-gamma. Belongs to the CD225/Dispanin family.

Protein type: Membrane protein, integral; Cell surface

Chromosomal Location of Human Ortholog: 11p15.5

Cellular Component: lysosomal membrane; late endosome membrane; integral to membrane; plasma membrane

Molecular Function: protein binding

Biological Process: negative regulation of viral transcription; negative regulation of viral genome replication; negative regulation of virion penetration into host cell; response to virus; cytokine and chemokine mediated signaling pathway; immune response; defense response to virus

Disease: Influenza, Severe, Susceptibility To