Rat CD63 antigen ELISA Kit | Cd63 elisa kit

Rat CD63 antigen ELISA Kit

Cat. Num. AAA2887498

• Reactivity: Rat
• Synonyms: CD63 antigen; Rat CD63 antigen ELISA Kit; Mast cell antigen AD1; Cd63; CD63; Cd63 elisa kit

• Reactivity: Rat
• Sequence Length: 238

• Samples: Serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
• Preparation and Storage: For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for Cd63 elisa kit

Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 8393076
• NCBI GeneID: 29186
• NCBI Accession #: NP_058821.1
• NCBI GenBank Nucleotide #: NM_017125.3
• UniProt Accession #: P28648
• Molecular Weight: 25,699 Da
• NCBI Official Full Name: CD63 antigen
• NCBI Official Synonym Full Names: Cd63 molecule
• NCBI Official Symbol: Cd63
• NCBI Protein Information: CD63 antigen
• UniProt Protein Name: CD63 antigen
• Protein Family: CD63 antigen
• UniProt Gene Name: Cd63
• UniProt Entry Name: CD63_RAT

NCBI Description

human homolog facilitates endocytosis of H,K-ATPase beta-subunit; may play a role in protein trafficking [RGD, Feb 2006]

Uniprot Description

Functions as cell surface receptor for TIMP1 and plays a role in the activation of cellular signaling cascades. Plays a role in the activation of ITGB1 and integrin signaling, leading to the activation of AKT, FAK/PTK2 and MAP kinases. Promotes cell survival, reorganization of the actin cytoskeleton, cell adhesion, spreading and migration, via its role in the activation of AKT and FAK/PTK2. Plays a role in VEGFA signaling via its role in regulating the internalization of KDR/VEGFR2. Plays a role in intracellular vesicular transport processes, and is required for normal trafficking of the PMEL luminal domain that is essential for the development and maturation of melanocytes. Plays a role in the adhesion of leukocytes onto endothelial cells via its role in the regulation of SELP trafficking. May play a role in mast cell degranulation in response to Ms4a2/FceRI stimulation, but not in mast cell degranulation in response to other stimuli ().