Human Carboxypeptidase M ELISA Kit | CPM elisa kit

Human Carboxypeptidase M ELISA Kit

Cat. Num. AAA2885667

• Reactivity: Human
• Synonyms: Carboxypeptidase M; Human Carboxypeptidase M ELISA Kit; CPM; 3.4.17.12; CPM elisa kit

• Reactivity: Human
• Specificity: Recombinant and natural Human Carboxypeptidase M,CPM
• Sequence Length: 443

• Samples: Serum, plasma, tissue homogenates, cell culture supernates or other biological fluids
• Detection Range: 1.56 ng/mL - 100 ng/mL
• Sensitivity: < 0.67 ng/mL
• Intra-assay Precision: <=5.8%
• Inter-assay Precision: <=10.9%
• Preparation and Storage: For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CPM elisa kit

Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 53832021
• NCBI GeneID: 1368
• NCBI Accession #: NP_001005502.1
• NCBI GenBank Nucleotide #: NM_001005502.2
• UniProt Accession #: P14384; Q9H2K9; B2R800
• Molecular Weight: 50,514 Da
• NCBI Official Full Name: carboxypeptidase M
• NCBI Official Synonym Full Names: carboxypeptidase M
• NCBI Official Symbol: CPM
• NCBI Protein Information: carboxypeptidase M
• UniProt Protein Name: Carboxypeptidase M
• Protein Family: Carboxypeptidase
• UniProt Gene Name: CPM
• UniProt Synonym Gene Names: CPM
• UniProt Entry Name: CBPM_HUMAN

NCBI Description

The protein encoded by this gene is a membrane-bound arginine/lysine carboxypeptidase. Its expression is associated with monocyte to macrophage differentiation. This encoded protein contains hydrophobic regions at the amino and carboxy termini and has 6 potential asparagine-linked glycosylation sites. The active site residues of carboxypeptidases A and B are conserved in this protein. Three alternatively spliced transcript variants encoding the same protein have been described for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

CPM: Specifically removes C-terminal basic residues (Arg or Lys) from peptides and proteins. It is believed to play important roles in the control of peptide hormone and growth factor activity at the cell surface, and in the membrane-localized degradation of extracellular proteins. Belongs to the peptidase M14 family.

Protein type: EC 3.4.17.12; Membrane protein, GPI anchor; Protease

Chromosomal Location of Human Ortholog: 12q14.3

Cellular Component: cell surface; extracellular space; plasma membrane

Molecular Function: carboxypeptidase activity; metallocarboxypeptidase activity; serine carboxypeptidase activity; zinc ion binding

Biological Process: anatomical structure morphogenesis; peptide metabolic process; protein processing