Rat Phosphatidylinositol-3,4,5-trisphosphate 5-phosphatase 1 ELISA Kit | Inpp5d elisa kit

Rat Phosphatidylinositol-3,4,5-trisphosphate 5-phosphatase 1 ELISA Kit

Cat. Num. AAA288462

• Reactivity: Rat
• Synonyms: Phosphatidylinositol-3; 4; 5-trisphosphate 5-phosphatase 1; Rat Phosphatidylinositol-3; 5-trisphosphate 5-phosphatase 1 ELISA Kit; Inpp5d elisa kit

• Reactivity: Rat
• Specificity: This assay recognizes recombinant and natural Rat Phosphatidylinositol-3,4,5-trisphosphate 5-phosphatase 1. No significant cross-reactivity or interference was observed.
• Sequence Length: 1190

• Assay Type: Competitive
• Detection Range: 1.56-100 ng/ml
• Sensitivity: 0.88 ng/mL
• Intra-Assay CV: <=5.1%
• Inter-Assay CV: <=8.9%
• Recovery: 89%
• Preparation and Storage: For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for Inpp5d elisa kit

Principle of the Assay: The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Phosphatidylinositol-3,4,5-trisphosphate 5-phosphatase 1, During the reaction, Phosphatidylinositol-3,4,5-trisphosphate 5-phosphatase 1 in the sample or standard competes with a fixed amount of biotin-labeled Phosphatidylinositol-3,4,5-trisphosphate 5-phosphatase 1 for sites on a pre-coated Monoclonal antibody specific to Phosphatidylinositol-3,4,5-trisphosphate 5-phosphatase 1. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Phosphatidylinositol-3,4,5-trisphosphate 5-phosphatase 1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 9506813
• NCBI GeneID: 54259
• NCBI Accession #: NP_062184.1
• NCBI GenBank Nucleotide #: NM_019311.1
• UniProt Accession #: P97573
• Molecular Weight: 133,593 Da
• NCBI Official Full Name: phosphatidylinositol 3,4,5-trisphosphate 5-phosphatase 1
• UniProt Protein Name: Phosphatidylinositol 3,4,5-trisphosphate 5-phosphatase 1
• Protein Family: Phosphatidylinositol 3,4,5-trisphosphate 5-phosphatase
• UniProt Gene Name: Inpp5d
• UniProt Synonym Gene Names: Ship; Ship1; SH2 domain-containing inositol phosphatase 1; SHIP-1
• UniProt Entry Name: SHIP1_RAT

Uniprot Description

SHIP: an SH2-containing inositol phosphatase. A hemopoietic-specific phosphatase that regulates cell survival, growth, cell cycle arrest and apoptosis. Hydrolyzes Ins(1,3,4,5)P4 and PtdIns(3,4,5)P3. A cytosolic protein with a SH2 domain in its N-terminus and two NPXY Shc binding motifs at its C-terminus. Upon receptor cross-linking, SHIP is first recruited to the plasma membrane by its SH2, followed by tyrosine phosphorylation on the NPXY motif. Both membrane relocalization and phosphorylation are essential for its regulatory function. Associates with SHC after cytokine stimulation.

Protein type: EC 3.1.3.86; Motility/polarity/chemotaxis; Phosphatase, lipid

Cellular Component: actin filament; cortical cytoskeleton; cytosol; intracellular; lipid raft; plasma membrane

Molecular Function: inositol-4,5-bisphosphate 5-phosphatase activity; inositol-polyphosphate 5-phosphatase activity; protein binding; PTB domain binding; SH3 domain binding

Biological Process: apoptosis; determination of adult life span; immune system process; immunoglobulin mediated immune response; negative regulation of B cell activation; negative regulation of B cell proliferation; negative regulation of bone resorption; negative regulation of cell proliferation; negative regulation of granulocyte differentiation; negative regulation of immune response; negative regulation of interleukin-6 biosynthetic process; negative regulation of monocyte differentiation; negative regulation of neutrophil differentiation; negative regulation of osteoclast differentiation; negative regulation of signal transduction; phosphoinositide dephosphorylation; positive regulation of apoptosis; positive regulation of B cell differentiation; positive regulation of erythrocyte differentiation; positive regulation of lymphocyte differentiation