Mouse ATP-binding cassette sub-family G member 2 ELISA Kit | Abcg2 elisa kit

Mouse ATP-binding cassette sub-family G member 2 ELISA Kit

Cat. Num. AAA288450

• Gene Names: Abcg2; MXR; ABCP; BCRP; MXR1; ABC15; Bcrp1; AI428558
• Reactivity: Mouse
• Synonyms: ATP-binding cassette sub-family G member 2; Mouse ATP-binding cassette sub-family G member 2 ELISA Kit; Abcg2 elisa kit

• Reactivity: Mouse
• Sequence Length: 658

• Assay Type: Competitive
• Detection Range: 0.312-20 ng/ml
• Sensitivity: 0.165 ng/mL
• Intra-Assay CV: <=7.6%
• Inter-Assay CV: <=10.1%
• Recovery: 87%
• Preparation and Storage: For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for Abcg2 elisa kit

Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

Principle of the Assay: The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen, During the reaction, target antigen in the sample or standard competes with a fixed amount of biotin-labeled target antigen for sites on a pre-coated antibody specific to target antigen. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve

NCBI and Uniprot Product Information

• NCBI GI #: 6752944
• NCBI GeneID: 26357
• NCBI Accession #: NP_036050.1
• NCBI GenBank Nucleotide #: NM_011920.3
• UniProt Accession #: Q7TMS5; Q9R004; Q9Z1T0
• Molecular Weight: 72,978 Da
• NCBI Official Full Name: ATP-binding cassette sub-family G member 2
• NCBI Official Synonym Full Names: ATP-binding cassette, sub-family G (WHITE), member 2
• NCBI Official Symbol: Abcg2
• NCBI Official Synonym Symbols: MXR; ABCP; BCRP; MXR1; ABC15; Bcrp1; AI428558
• NCBI Protein Information: ATP-binding cassette sub-family G member 2
• UniProt Protein Name: ATP-binding cassette sub-family G member 2
• Protein Family: ABC transporter G family
• UniProt Gene Name: Abcg2
• UniProt Synonym Gene Names: Abcp; Bcrp1
• UniProt Entry Name: ABCG2_MOUSE

NCBI Description

The membrane-associated protein encoded by this gene is included in the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the White subfamily. Alternatively referred to as a breast cancer resistance protein, the human protein functions as a xenobiotic transporter which may play a major role in multi-drug resistance. This protein likely serves as a cellular defense mechanism in response to mitoxantrone and anthracycline exposure. [provided by RefSeq, Jul 2008]

Uniprot Description

ABCG2: Xenobiotic transporter that may play an important role in the exclusion of xenobiotics from the brain. May be involved in brain-to-blood efflux. Appears to play a major role in the multidrug resistance phenotype of several cancer cell lines. When overexpressed, the transfected cells become resistant to mitoxantrone, daunorubicin and doxorubicin, display diminished intracellular accumulation of daunorubicin, and manifest an ATP- dependent increase in the efflux of rhodamine 123. Monomer or homodimer; disulfide-linked. Up-regulated in brain tumors. Highly expressed in placenta. Low expression in small intestine, liver and colon. Belongs to the ABC transporter superfamily. ABCG family. Eye pigment precursor importer (TC 3.A.1.204) subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transporter; Membrane protein, integral; Transporter, iron; Transporter, ABC family; Membrane protein, multi-pass

Cellular Component: apical plasma membrane; integral to membrane; membrane; mitochondrion; nucleus; plasma membrane

Molecular Function: ATP binding; ATPase activity; ATPase activity, coupled to transmembrane movement of substances; drug transporter activity; nucleotide binding; protein dimerization activity; protein homodimerization activity

Biological Process: drug export; drug transport; embryonic process involved in female pregnancy; multidrug transport; transmembrane transport; transport; urate metabolic process