Mouse NLR family CARD domain-containing protein 4 ELISA Kit | Nlrc4 elisa kit

Mouse NLR family CARD domain-containing protein 4 ELISA Kit

Cat. Num. AAA288342

• Gene Names: Nlrc4; CLAN; IPAF; CLAN1; CLANA; CLANB; CLANC; CLAND; Card12; 9530011P19Rik
• Reactivity: Mouse
• Synonyms: NLR family CARD domain-containing protein 4; Mouse NLR family CARD domain-containing protein 4 ELISA Kit; Nlrc4 elisa kit

• Reactivity: Mouse
• Specificity: This assay recognizes recombinant and natural mouse NLR family CARD domain-containing protein 4. No significant cross-reactivity or interference was observed.
• Sequence Length: 1024

• Assay Type: Sandwich
• Detection Range: 0.156-10 ng/ml
• Sensitivity: 0.083 ng/mL
• Intra-Assay CV: <=5.1%
• Inter-Assay CV: <=8.4%
• Recovery: 101%
• Preparation and Storage: For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for Nlrc4 elisa kit

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to NLR family CARD domain-containing protein 4. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for NLR family CARD domain-containing protein 4 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain NLR family CARD domain-containing protein 4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of NLR family CARD domain-containing protein 4 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 77736546
• NCBI GeneID: 268973
• NCBI Accession #: NP_001028539.1
• NCBI GenBank Nucleotide #: NM_001033367.3
• UniProt Accession #: Q3UP24; Q3TAU8
• Molecular Weight: 116,749 Da
• NCBI Official Full Name: NLR family CARD domain-containing protein 4
• NCBI Official Synonym Full Names: NLR family, CARD domain containing 4
• NCBI Official Symbol: Nlrc4
• NCBI Official Synonym Symbols: CLAN; IPAF; CLAN1; CLANA; CLANB; CLANC; CLAND; Card12; 9530011P19Rik
• NCBI Protein Information: NLR family CARD domain-containing protein 4
• UniProt Protein Name: NLR family CARD domain-containing protein 4
• Protein Family: NLR family CARD domain-containing protein
• UniProt Gene Name: Nlrc4
• UniProt Synonym Gene Names: Card12; Ipaf; Ipaf
• UniProt Entry Name: NLRC4_MOUSE

Uniprot Description

NLRC4: Key component of inflammasomes that indirectly senses specific proteins from pathogenic bacteria and fungi and responds by assembling an inflammasome complex that promotes caspase-1 activation, cytokine production and macrophage pyroptosis. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Apoptosis

Cellular Component: cytoplasm; cytosol; nucleus; spindle microtubule

Molecular Function: ATP binding; cysteine protease inhibitor activity; identical protein binding; nucleotide binding; protein homodimerization activity; ubiquitin-protein ligase activity

Biological Process: activation of innate immune response; activation of NF-kappaB transcription factor; apoptosis; caspase activation; defense response to bacterium; detection of bacterium; immune system process; inflammatory response; innate immune response; interleukin-1 beta secretion; positive regulation of apoptosis; protein homooligomerization; regulation of apoptosis; regulation of caspase activity; regulation of signal transduction