Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Phosphatidylethanolamine-binding protein 1 (PEBP1) Standard Curve.)
Human Phosphatidylethanolamine-Binding Protein 1 (PEBP1) ELISA Kit | PEBP1 elisa kit
Human Phosphatidylethanolamine-Binding Protein 1 (PEBP1) ELISA Kit
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Phosphatidylethanolamine-binding protein 1 (PEBP1) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Phosphatidylethanolamine-binding protein 1 (PEBP1) Standard Curve.)
Background: RKIP cDNA encodes a protein of 186 amino acids, the sequence of which is 95% identical to the bovine 21- to 23-kD protein. The rat protein shows 85.5% identity with the human protein. highly expressed in the brain and associated with cytosolic proteins and small GTP-binding proteins. Multiple tissue Northern blots revealed the presence of a single mRNA in the different tissues of each species; a single band of 1.8 kb was identified in human, 1.45 kb in mouse, and 1.2 kb in rat tissues. The mRNA is particularly highly expressed in rat and mouse testis, where the level was 30 times higher than that in the brain. PBP is expressed strongly in the cell bodies of oligodendrocytes but only weakly elsewhere. Schwann cells and spinal nerve roots showed intense cytoplasmic PBP immunoreactivity.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the phosphatidylethanolamine-binding family of proteins and has been shown to modulate multiple signaling pathways, including the MAP kinase (MAPK), NF-kappa B, and glycogen synthase kinase-3 (GSK-3) signaling pathways. The encoded protein can be further processed to form a smaller cleavage product, hippocampal cholinergic neurostimulating peptide (HCNP), which may be involved in neural development. This gene has been implicated in numerous human cancers and may act as a metastasis suppressor gene. Multiple pseudogenes of this gene have been identified in the genome. [provided by RefSeq, Jul 2015]
Uniprot Description
RKIP: a metastasis suppressor that regulates extracellular matrix remodeling and tumor survival. Binds to and inhibits Raf-1. Phosphorylation by classic and atypical but not novel PKC isoforms dissociates this complex, relieving inhibition of the Raf/MAP kinase signaling cascade. Binds ATP, opioids and phosphatidylethanolamine. Has lower affinity for phosphatidylinositol and phosphatidylcholine. Serine protease inhibitor which inhibits thrombin, neuropsin and chymotrypsin but not trypsin, tissue type plasminogen activator and elastase Inhibits the kinase activity of RAF1 by inhibiting its activation and by dissociating the RAF1/MEK complex and acting as a competitive inhibitor of MEK phosphorylation. Cleaved into hippocampal cholinergic neurostimulating peptide (HCMP) that appears to be involved in the function of the presynaptic cholinergic neurons of the central nervous system. HCNP increases the production of choline acetyltransferase but not acetylcholinesterase. Expressed in brain. Increased expression in aged senescence-accelerated mice.
Protein type: Protein kinase, regulatory subunit; Lipid-binding
Chromosomal Location of Human Ortholog: 12q24.23
Cellular Component: cytoplasm; nucleus
Molecular Function: serine-type endopeptidase inhibitor activity; protein binding; enzyme binding; phosphatidylethanolamine binding; protein kinase binding; ATP binding
Research Articles on PEBP1
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Product Notes
The Human PEBP1 pebp1 (Catalog #AAA9714921) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9714921 ELISA Kit recognizes Human PEBP1. It is sometimes possible for the material contained within the vial of "Phosphatidylethanolamine-Binding Protein 1 (PEBP1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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