Salmon Follitropin subunit beta ELISA Kit | FSHB elisa kit

Salmon Follitropin subunit beta ELISA Kit

Cat. Num. AAA288256

• Gene Names: FSHB; HH24
• Reactivity: Salmon
• Synonyms: Follitropin subunit beta; Salmon Follitropin subunit beta ELISA Kit; FSHB elisa kit

• Reactivity: Salmon
• Specificity: This assay recognizes recombinant and natural salmon Follitropin subunit beta. No significant cross-reactivity or interference was observed.
• Sequence Length: 129

• Samples: Serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
• Detection Range: 0.15 mIU/mL -10.0 mIU/mL.
• Sensitivity: The minimum detectable dose of salmon Follitropin subunit beta is typically less than 0.039 mIU/mL.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero.
• Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of salmon Follitropin subunit beta concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
• Preparation and Storage: For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for FSHB elisa kit

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to Follitropin subunit beta. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Follitropin subunit beta and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Follitropin subunit beta, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Follitropin subunit beta in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 66528995
• NCBI GeneID: 2488
• NCBI Accession #: NP_001018090.1
• NCBI GenBank Nucleotide #: NM_001018080.1
• Molecular Weight: 14,700 Da
• NCBI Official Full Name: follitropin subunit beta
• NCBI Official Synonym Full Names: follicle stimulating hormone beta subunit
• NCBI Official Symbol: FSHB
• NCBI Official Synonym Symbols: HH24
• NCBI Protein Information: follitropin subunit beta
• UniProt Protein Name: Follitropin subunit beta
• Protein Family: Follitropin
• UniProt Gene Name: FSHB
• UniProt Synonym Gene Names: FSH-B; FSH-beta
• UniProt Entry Name: FSHB_HUMAN

NCBI Description

The pituitary glycoprotein hormone family includes follicle-stimulating hormone, luteinizing hormone, chorionic gonadotropin, and thyroid-stimulating hormone. All of these glycoproteins consist of an identical alpha subunit and a hormone-specific beta subunit. This gene encodes the beta subunit of follicle-stimulating hormone. In conjunction with luteinizing hormone, follicle-stimulating hormone induces egg and sperm production. Alternative splicing results in two transcript variants encoding the same protein. [provided by RefSeq, Jul 2008]

Uniprot Description

FSHB: Stimulates development of follicle and spermatogenesis in the reproductive organs. Defects in FSHB are a cause of isolated follicle- stimulating hormone deficiency (IFSHD). Selective follicle-stimulating hormone deficiency is an uncommon cause of infertility, producing amenorrhea and hypogonadism in women and oligo or azoospermia with normal testosterone levels in normally virilised men. Belongs to the glycoprotein hormones subunit beta family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 11p13

Cellular Component: cytoplasm; extracellular region

Molecular Function: follicle-stimulating hormone activity; hormone activity; protein binding

Biological Process: cellular protein metabolic process; female gamete generation; female pregnancy; follicle-stimulating hormone signaling pathway; peptide hormone processing; positive regulation of bone resorption; positive regulation of cell migration; positive regulation of cell proliferation; positive regulation of transcription from RNA polymerase II promoter; progesterone biosynthetic process; regulation of osteoclast differentiation; Sertoli cell proliferation; signal transduction; transforming growth factor beta receptor signaling pathway

Disease: Follicle-stimulating Hormone Deficiency, Isolated