Typical Testing Data/Standard Curve (for reference only)
Rat DNA fragmentation factor subunit beta ELISA Kit | Dffb elisa kit
Rat DNA fragmentation factor subunit beta ELISA Kit
Gene Names
Dffb; Cad
Reactivity
Rat
Synonyms
DNA fragmentation factor subunit beta; Rat DNA fragmentation factor subunit beta ELISA Kit; Caspase-activated deoxyribonuclease; CAD; Caspase-activated DNase; DNA fragmentation factor 40 kDa subunit; DFF-40; Dffb; Cad; Dffb elisa kit
Reactivity
Rat
Specificity
Recombinant and natural Rat DNA fragmentation factor subunit beta
Sequence Length
349
Samples
Serum, plasma, tissue homogenates, cell culture supernates or other biological fluids
Detection Range
15.6-1000 pg/mL
Sensitivity
<8.1 pg/mL
Intra-assay Precision
<=5.1%
Inter-assay Precision
<=9.8%
Preparation and Storage
For long term storage, please store the entire kit at -20 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for Dffb elisa kit
Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
40,097 Da
NCBI Official Full Name
DNA fragmentation factor subunit beta
NCBI Official Synonym Full Names
DNA fragmentation factor subunit beta
NCBI Official Symbol
Dffb
NCBI Official Synonym Symbols
Cad
NCBI Protein Information
DNA fragmentation factor subunit beta
UniProt Protein Name
DNA fragmentation factor subunit beta
Protein Family
UniProt Gene Name
Dffb
UniProt Synonym Gene Names
Cad; CAD; Caspase-activated DNase; DFF-40
UniProt Entry Name
DFFB_RAT
NCBI Description
endogenous endonuclease; mediates caspase-3-dependent internucleosomal DNA degradation and related nuclear alterations [RGD, Feb 2006]
Uniprot Description
DFFB: Nuclease that induces DNA fragmentation and chromatin condensation during apoptosis. Degrades naked DNA and induces apoptotic morphology. 4 isoforms of the human protein are produced by alternative splicing.
Protein type: DNA-binding; Transferase; Apoptosis; Deoxyribonuclease; Hydrolase; EC 3.-.-.-
Cellular Component: cytoplasm; cytosol; nuclear chromatin; nucleolus; nucleus
Molecular Function: deoxyribonuclease activity; DNA binding; enzyme binding; nuclease activity; protein binding
Biological Process: apoptotic chromosome condensation; brain development; DNA fragmentation during apoptosis
Research Articles on Dffb
Similar Products
Product Notes
The Rat Dffb dffb (Catalog #AAA2882336) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2882336 ELISA Kit recognizes Rat Dffb. It is sometimes possible for the material contained within the vial of "DNA fragmentation factor subunit beta, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
