Rat Mothers against decapentaplegic homolog 3 ELISA Kit | Smad3 elisa kit

Rat Mothers against decapentaplegic homolog 3 ELISA Kit

Cat. Num. AAA2882277

• Gene Names: Smad3; mad3; Madh3; Smad 3
• Reactivity: Rat
• Synonyms: Mothers against decapentaplegic homolog 3; Rat Mothers against decapentaplegic homolog 3 ELISA Kit; MAD homolog 3; Mad3; Mothers against DPP homolog 3; SMAD family member 3; SMAD 3; Smad3; Madh3; Smad3 elisa kit

• Reactivity: Rat
• Specificity: Recombinant and natural Rat Mothers against decapentaplegic homolog 3,MAD homolog 3
• Sequence Length: 425

• Samples: Serum, plasma, tissue homogenates, cell culture supernates or other biological fluids
• Detection Range: 0.312-20 ng/mL
• Sensitivity: <0.196 ng/mL
• Intra-assay Precision: Intra-Assay CV: <=4.2%
• Inter-assay Precision: Inter-Assay CV: <=7.1%
• Preparation and Storage: For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for Smad3 elisa kit

Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 6981174
• NCBI GeneID: 25631
• NCBI Accession #: NP_037227.1
• NCBI GenBank Nucleotide #: NM_013095.3
• UniProt Accession #: P84025; O09064; O09144; O14510; O35273; Q92940; Q93002; Q9GKR4
• Molecular Weight: 48,081 Da
• NCBI Official Full Name: mothers against decapentaplegic homolog 3
• NCBI Official Synonym Full Names: SMAD family member 3
• NCBI Official Symbol: Smad3
• NCBI Official Synonym Symbols: mad3; Madh3; Smad 3
• NCBI Protein Information: mothers against decapentaplegic homolog 3
• UniProt Protein Name: Mothers against decapentaplegic homolog 3
• Protein Family: Mothers against decapentaplegic
• UniProt Gene Name: Smad3
• UniProt Synonym Gene Names: Madh3; MAD homolog 3; Mad3; Mothers against DPP homolog 3; SMAD 3; Smad3
• UniProt Entry Name: SMAD3_RAT

NCBI Description

transcription factor; important component of the TGF-beta signaling pathway [RGD, Feb 2006]

Uniprot Description

SMAD3: transcription factor phosphorylated and activated by TGF-beta-type receptors. A receptor-regulated Smad (R-smad). Binds directly to consensus DNA-binding elements in the promoters of target genes. In mouse required for establishemnt of the mucosal immune response and proper development of skeleton.

Protein type: Nuclear receptor co-regulator; Transcription factor; DNA-binding

Cellular Component: cytoplasm; nuclear chromatin; nuclear inner membrane; nucleoplasm; nucleus; plasma membrane; protein complex; receptor complex; transcription factor complex

Molecular Function: beta-catenin binding; bHLH transcription factor binding; chromatin binding; chromatin DNA binding; collagen binding; DNA binding; double-stranded DNA binding; enzyme binding; identical protein binding; phosphatase binding; protein binding; protein heterodimerization activity; protein homodimerization activity; protein kinase binding; sequence-specific DNA binding; SMAD binding; transcription factor activity; transcription factor binding; transforming growth factor beta receptor binding; transforming growth factor beta receptor, pathway-specific cytoplasmic mediator activity; ubiquitin binding; ubiquitin protein ligase binding; zinc ion binding

Biological Process: activin receptor signaling pathway; caspase activation; cell cycle arrest; developmental growth; embryonic cranial skeleton morphogenesis; embryonic foregut morphogenesis; embryonic pattern specification; endoderm development; evasion of host defenses by virus; gastrulation; heart looping; immune response; immune system development; in utero embryonic development; intercellular junction assembly and maintenance; liver development; mesoderm formation; negative regulation of apoptosis; negative regulation of cell growth; negative regulation of cell proliferation; negative regulation of fat cell differentiation; negative regulation of inflammatory response; negative regulation of mitotic cell cycle; negative regulation of osteoblast differentiation; negative regulation of osteoblast proliferation; negative regulation of protein amino acid phosphorylation; negative regulation of protein catabolic process; negative regulation of transcription from RNA polymerase II promoter; osteoblast development; osteoblast differentiation; paraxial mesoderm morphogenesis; pericardium development; positive regulation of bone mineralization; positive regulation of cell migration; positive regulation of chondrocyte differentiation; positive regulation of focal adhesion formation; positive regulation of interleukin-1 beta production; positive regulation of positive chemotaxis; positive regulation of stress fiber formation; positive regulation of transcription factor import into nucleus; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; positive regulation of transforming growth factor-beta3 production; protein stabilization; regulation of binding; regulation of epithelial cell proliferation; regulation of immune response; regulation of striated muscle development; regulation of transcription from RNA polymerase II promoter; regulation of transforming growth factor beta receptor signaling pathway; regulation of transforming growth factor-beta2 production; response to hypoxia; skeletal development; SMAD protein complex assembly; somitogenesis; T cell activation; thyroid gland development; transcription, DNA-dependent; transforming growth factor beta receptor signaling pathway; transport; ureteric bud development