Human Periostin ELISA Kit | POSTN elisa kit

Human Periostin ELISA Kit

Cat. Num. AAA2882096

• Gene Names: POSTN; PN; OSF2; OSF-2; PDLPOSTN
• Reactivity: Human
• Synonyms: Periostin; Human Periostin ELISA Kit; PN; Osteoblast-specific factor 2; OSF-2; POSTN; OSF2; POSTN elisa kit

• Reactivity: Human
• Specificity: Recombinant and natural Human Periostin,PN
• Sequence Length: 779

• Samples: Serum, plasma, tissue homogenates, cell culture supernates or other biological fluids
• Detection Range: 15.6-1000 ng/mL
• Sensitivity: <9.8 ng/mL
• Intra-assay Precision: Intra-Assay CV: <=6.9%
• Inter-assay Precision: Inter-Assay CV: <=8.7%
• Preparation and Storage: For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for POSTN elisa kit

Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 209862911
• NCBI GeneID: 10631
• NCBI Accession #: NP_001129406.1
• NCBI GenBank Nucleotide #: NM_001135934.1
• UniProt Accession #: Q15063; Q15064; Q29XZ0; Q3KPJ5; Q5VSY5; Q8IZF9; B1ALD8; C0IMJ1; C0IMJ2; C0IMJ4; D2KRH7; F5H628
• Molecular Weight: 80,357 Da
• NCBI Official Full Name: periostin isoform 2
• NCBI Official Synonym Full Names: periostin, osteoblast specific factor
• NCBI Official Symbol: POSTN
• NCBI Official Synonym Symbols: PN; OSF2; OSF-2; PDLPOSTN
• NCBI Protein Information: periostin
• UniProt Protein Name: Periostin
• Protein Family: Periostin
• UniProt Gene Name: POSTN
• UniProt Synonym Gene Names: OSF2; PN; OSF-2
• UniProt Entry Name: POSTN_HUMAN

NCBI Description

This gene encodes a secreted extracellular matrix protein that functions in tissue development and regeneration, including wound healing, and ventricular remodeling following myocardial infarction. The encoded protein binds to integrins to support adhesion and migration of epithelial cells. This protein plays a role in cancer stem cell maintenance and metastasis. Mice lacking this gene exhibit cardiac valve disease, and skeletal and dental defects. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Sep 2015]

Uniprot Description

POSTN: Binds to heparin. Induces cell attachment and spreading and plays a role in cell adhesion. May play a role in extracellular matrix mineralization. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide; Cell adhesion; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 13q13.3

Cellular Component: extracellular matrix; extracellular space; neuromuscular junction; proteinaceous extracellular matrix; trans-Golgi network

Molecular Function: cell adhesion molecule binding; heparin binding; protein binding

Biological Process: cell adhesion; extracellular matrix organization and biogenesis; negative regulation of cell-matrix adhesion; positive regulation of smooth muscle cell migration; regulation of Notch signaling pathway; regulation of systemic arterial blood pressure; response to estradiol stimulus; response to hypoxia; response to mechanical stimulus; response to muscle activity; skeletal development