Chicken V(D)J recombination-activating protein 2 ELISA Kit | RAG2 elisa kit

Chicken V(D)J recombination-activating protein 2 ELISA Kit

Cat. Num. AAA288203

• Gene Names: RAG2; Rag-2
• Reactivity: Chicken
• Synonyms: V(D)J recombination-activating protein 2; Chicken V(D)J recombination-activating protein 2 ELISA Kit; RAG2 elisa kit

• Reactivity: Chicken
• Specificity: This assay recognizes recombinant and natural chicken RAG-2. No significant cross-reactivity or interference was observed.
• Sequence Length: 527

• Samples: Serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
• Detection Range: 0.78 ng/ml -50.0 ng/ml.
• Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of chicken RAG-2 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
• Preparation and Storage: For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for RAG2 elisa kit

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to RAG-2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for RAG-2 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain RAG-2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of RAG-2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 131830
• NCBI GeneID: 423165
• UniProt Accession #: P25022
• Molecular Weight: 59,089 Da
• NCBI Official Full Name: V(D)J recombination-activating protein 2
• NCBI Official Synonym Full Names: recombination activating gene 2
• NCBI Official Symbol: RAG2
• NCBI Official Synonym Symbols: Rag-2
• NCBI Protein Information: V(D)J recombination-activating protein 2
• UniProt Protein Name: V(D)J recombination-activating protein 2
• Protein Family: V(D)J recombination-activating protein
• UniProt Gene Name: RAG2
• UniProt Synonym Gene Names: RAG-2
• UniProt Entry Name: RAG2_CHICK

Uniprot Description

Core component of the RAG complex, a multiprotein complex that mediates the DNA cleavage phase during V(D)J recombination. V(D)J recombination assembles a diverse repertoire of immunoglobulin and T-cell receptor genes in developing B and T lymphocytes through rearrangement of different V (variable), in some cases D (diversity), and J (joining) gene segments. DNA cleavage by the RAG complex occurs in 2 steps: a first nick is introduced in the top strand immediately upstream of the heptamer, generating a 3'-hydroxyl group that can attack the phosphodiester bond on the opposite strand in a direct transesterification reaction, thereby creating 4 DNA ends: 2 hairpin coding ends and 2 blunt, 5'-phosphorylated ends. In the RAG complex, RAG2 is not the catalytic component but is required for all known catalytic activities mediated by RAG1. It probably acts as a sensor of chromatin state that recruits the RAG complex to H3K4me3 ().