Typical Testing Data/Standard Curve (for reference only)
Rat Cytochrome P450 19A1 ELISA Kit | Cyp19a1 elisa kit
Rat Cytochrome P450 19A1 ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI Description
catalyzes end-step estrogen formation from androgens and aromatization of testosterone to estradiol [RGD, Feb 2006]
Uniprot Description
CYP19A1: Catalyzes the formation of aromatic C18 estrogens from C19 androgens. Belongs to the cytochrome P450 family.
Protein type: Lipid Metabolism - androgen and estrogen; Oxidoreductase; EC 1.14.14.14
Cellular Component: cell soma; cytoplasm; cytosol; dendritic spine; endoplasmic reticulum; membrane; nerve terminal; synapse; synaptic vesicle; terminal button
Molecular Function: heme binding; iron ion binding; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen
Biological Process: adrenal gland development; androgen metabolic process; bile acid metabolic process; biphenyl metabolic process; C21-steroid hormone metabolic process; dibenzo-p-dioxin metabolic process; estrogen biosynthetic process; granulocyte differentiation; hippocampus development; hypothalamus development; luteinization; male gonad development; melatonin metabolic process; negative regulation of bone resorption; negative regulation of chronic inflammatory response; phthalate metabolic process; regulation of synapse structure and activity; response to cesium; response to drug; response to estradiol stimulus; response to estrogen stimulus; response to herbicide; response to ionizing radiation; response to metal ion; response to morphine; response to organic cyclic substance; response to testosterone stimulus
Research Articles on Cyp19a1
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Product Notes
The Rat Cyp19a1 cyp19a1 (Catalog #AAA2881046) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2881046 ELISA Kit recognizes Rat Cyp19a1. It is sometimes possible for the material contained within the vial of "Cytochrome P450 19A1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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