Mouse Thioredoxin reductase 1, cytoplasmic ELISA Kit | Txnrd1 elisa kit

Mouse Thioredoxin reductase 1, cytoplasmic ELISA Kit

Cat. Num. AAA288050

• Gene Names: Txnrd1; TR; TR1; TrxR1
• Reactivity: Mouse
• Synonyms: Thioredoxin reductase 1; cytoplasmic; Mouse Thioredoxin reductase 1; cytoplasmic ELISA Kit; Txnrd1 elisa kit

• Reactivity: Mouse
• Specificity: This assay recognizes recombinant and natural mouse TR. No significant cross-reactivity or interference was observed.
• Sequence Length: 551

• Assay Type: Sandwich
• Detection Range: 0.156-10 ng/ml
• Sensitivity: 0.091 ng/mL
• Intra-Assay CV: <=5.1%
• Inter-Assay CV: <=7.6%
• Recovery: 107%
• Preparation and Storage: For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for Txnrd1 elisa kit

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to TR. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for TR and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain TR, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of TR in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 110224442
• NCBI GeneID: 50493
• NCBI Accession #: NP_001035978.1
• NCBI GenBank Nucleotide #: NM_001042513.1
• UniProt Accession #: Q9JMH6; Q3UEB7; Q3UK84; Q8CI31; Q99P49; Q9CSV5
• Molecular Weight: 54,544 Da
• NCBI Official Full Name: thioredoxin reductase 1, cytoplasmic isoform 2
• NCBI Official Synonym Full Names: thioredoxin reductase 1
• NCBI Official Symbol: Txnrd1
• NCBI Official Synonym Symbols: TR; TR1; TrxR1
• NCBI Protein Information: thioredoxin reductase 1, cytoplasmic
• UniProt Protein Name: Thioredoxin reductase 1, cytoplasmic
• Protein Family: Thioredoxin reductase
• UniProt Gene Name: Txnrd1
• UniProt Synonym Gene Names: Trxr1; TR
• UniProt Entry Name: TRXR1_MOUSE

NCBI Description

This gene encodes a member of the family of pyridine nucleotide-disulfide oxidoreductases. This protein is a flavoenzyme, which uses NADPH for reduction of thioredoxins as well as other protein and nonprotein substrates, and plays a role in protection against oxidative stress. It contains a selenocysteine (Sec) residue, which is essential for catalytic activity. The selenocysteine is encoded by the UGA codon that normally signals translation termination. The 3' UTR of Sec-containing genes have a common stem-loop structure, the sec insertion sequence (SECIS), that is necessary for the recognition of UGA as a Sec codon, rather than as a stop signal. Alternative splicing of this gene results in several transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

TRXR1: a cytoplasmic pyridine nucleotide oxidoreductases. This protein reduces thioredoxins as well as other substrates, and plays a role in selenium metabolism and protection against oxidative stress. The functional enzyme is thought to be a homodimer which uses FAD as a cofactor. Each subunit contains a selenocysteine residue which is required for the catalytic activity.

Protein type: Oxidoreductase; Nucleotide Metabolism - pyrimidine; EC 1.8.1.9; Nuclear receptor co-regulator

Cellular Component: cell soma; cytoplasm; cytosol; mitochondrion; nucleus

Molecular Function: FAD binding; mercury ion binding; NAD(P)H oxidase activity; oxidoreductase activity; oxidoreductase activity, acting on sulfur group of donors, NAD or NADP as acceptor; protein homodimerization activity; selenate reductase activity; thioredoxin-disulfide reductase activity

Biological Process: benzene and derivative metabolic process; cell proliferation; cell redox homeostasis; gastrulation; hydrogen peroxide catabolic process; mesoderm formation; protein tetramerization; response to oxidative stress; selenocysteine metabolic process