Rat Myelin proteolipid protein ELISA Kit | Plp1 elisa kit

Rat Myelin proteolipid protein ELISA Kit

Cat. Num. AAA2880148

• Gene Names: Plp1; Plp
• Reactivity: Rat
• Synonyms: Myelin proteolipid protein; Rat Myelin proteolipid protein ELISA Kit; PLP; Lipophilin; Plp1; Plp; Plp1 elisa kit

• Reactivity: Rat
• Sequence Length: 277

• Samples: Serum, plasma, tissue homogenates, cell culture supernates or other biological fluids
• Preparation and Storage: For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for Plp1 elisa kit

Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 13591880
• NCBI GeneID: 24943
• NCBI Accession #: NP_112252.1
• NCBI GenBank Nucleotide #: NM_030990.2
• UniProt Accession #: P60203; P04400; P06905; Q561K5
• Molecular Weight: 30,077 Da
• NCBI Official Full Name: myelin proteolipid protein
• NCBI Official Synonym Full Names: proteolipid protein 1
• NCBI Official Symbol: Plp1
• NCBI Official Synonym Symbols: Plp
• NCBI Protein Information: myelin proteolipid protein
• UniProt Protein Name: Myelin proteolipid protein
• Protein Family: Myelin proteolipid protein
• UniProt Gene Name: Plp1
• UniProt Synonym Gene Names: Plp; PLP
• UniProt Entry Name: MYPR_RAT

Uniprot Description

PLP1: This is the major myelin protein from the central nervous system. It plays an important role in the formation or maintenance of the multilamellar structure of myelin. Defects in PLP1 are the cause of leukodystrophy hypomyelinating type 1 (HLD1); also known as Pelizaeus-Merzbacher disease. HLD1 is an X-linked recessive dysmyelinating disorder of the central nervous system in which myelin is not formed properly. It is characterized clinically by nystagmus, spastic quadriplegia, ataxia, and developmental delay. Defects in PLP1 are the cause of spastic paraplegia X- linked type 2 (SPG2). SPG2 is characterized by spastic gait and hyperreflexia. In some patients, complicating features include nystagmus, dysarthria, sensory disturbance, mental retardation, optic atrophy. Belongs to the myelin proteolipid protein family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, multi-pass; Membrane protein, integral; Cell surface

Cellular Component: integral to plasma membrane; myelin sheath; plasma membrane

Molecular Function: structural constituent of myelin sheath; structural molecule activity

Biological Process: astrocyte development; axon ensheathment; cell maturation; glial cell differentiation; inflammatory response; integrin-mediated signaling pathway; long-chain fatty acid biosynthetic process; myelination; myelination in the central nervous system; substantia nigra development