Typical Testing Data/Standard Curve
Human Pepsin A-3 ELISA Kit | PGA3 elisa kit
Human Pepsin A-3 ELISA Kit
Gene Names
PGA5; Pg5
Reactivity
Human
Synonyms
Pepsin A-3; Human Pepsin A-3 ELISA Kit; Pepsin A; PGA3; PGA3 elisa kit
Reactivity
Human
Specificity
This assay recognizes recombinant and natural human Pepsin A. No significant cross-reactivity or interference was observed.
Assay Type
Sandwich
Detection Range
1.56-100 ng/mL
Sensitivity
0.78 ng/mL
Intra-Assay CV
<=4.5%
Inter-Assay CV
<=8.7%
Recovery
94%
Preparation and Storage
For long term storage, please store the entire kit at -20 degree C.
Typical Testing Data/Standard Curve
Typical Testing Data/Standard Curve
Related Product Information for PGA3 elisa kit
Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of human Pepsin A concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Principle of the Assay||The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pepsin A. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Pepsin A and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Pepsin A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Pepsin A in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay||The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pepsin A. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Pepsin A and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Pepsin A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Pepsin A in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a protein precursor of the digestive enzyme pepsin, a member of the peptidase A1 family of endopeptidases. The encoded precursor is secreted by gastric chief cells and undergoes autocatalytic cleavage in acidic conditions to form the active enzyme, which functions in the digestion of dietary proteins. This gene is found in a cluster of related genes on chromosome 11, each of which encodes one of multiple pepsinogens. Pepsinogen levels in serum may serve as a biomarker for atrophic gastritis and gastric cancer. [provided by RefSeq, Jul 2015]
Research Articles on PGA3
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Product Notes
The Human PGA3 (Catalog #AAA2880091) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2880091 ELISA Kit recognizes Human PGA3. It is sometimes possible for the material contained within the vial of "Pepsin A-3, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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