Canine Aminopeptidase N (ANPEP) ELISA Kit | ANPEP elisa kit

Canine Aminopeptidase N (ANPEP) ELISA Kit

Cat. Num. AAA287832

• Gene Names: ANPEP; APN
• Reactivity: Canine
• Synonyms: Aminopeptidase N (ANPEP); Canine Aminopeptidase N (ANPEP) ELISA Kit; APN; CD13; GP150; LAP1; P150; PEPN; aminopeptidase M; aminopeptidase N; membrane alanine aminopeptidase; microsomal aminopeptidase; myeloid plasma membrane glycoprotein CD13; ANPEP elisa kit

• Reactivity: Canine
• Specificity: This assay has high sensitivity and excellent specificity for detection of Canine ANPEP. No significant cross-reactivity or interference between Canine ANPEP and analogues was observed.
• Sequence Length: 975

• Assay Type: Quantitive Sandwich
• Samples: Serum, Plasma, Other Biological Fluids
• Detection Wavelength: 450 nm
• Intra-assay Precision (Precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
• Inter-assay Precision (Precision between assays): Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
• CV (%): = SD/meanX100
• Intra-Assay: CV
• Inter-Assay: CV
• Preparation and Storage: Store at 2-8 degree C.

Related Product Information for ANPEP elisa kit

This assay employs a two-site sandwich ELISA to quantitate ANPEP in samples. An antibody specific for ANPEP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ANPEP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ANPEP is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ANPEP bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 225637546
• NCBI GeneID: 403913
• NCBI Accession #: NP_001139506.1
• NCBI GenBank Nucleotide #: NM_001146034.1
• UniProt Accession #: P79143; F1PCB5
• Molecular Weight: 110,257 Da
• NCBI Official Full Name: aminopeptidase N
• NCBI Official Symbol: ANPEP
• NCBI Official Synonym Symbols: APN
• NCBI Protein Information: aminopeptidase N
• UniProt Protein Name: Aminopeptidase N
• Protein Family: Aminopeptidase
• UniProt Gene Name: ANPEP
• UniProt Synonym Gene Names: AP-N; cAPN; AP-M

Uniprot Description

Broad specificity aminopeptidase which plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases. Also involved in the processing of various peptides including peptide hormones, such as angiotensin III and IV, neuropeptides, and chemokines. May also be involved the cleavage of peptides bound to major histocompatibility complex class II molecules of antigen presenting cells. May have a role in angiogenesis and promote cholesterol crystallization.