Mouse MAP/microtubule affinity-regulating kinase 4 (MARK4) ELISA Kit | MARK4 elisa kit

Mouse MAP/microtubule affinity-regulating kinase 4 (MARK4) ELISA Kit

Cat. Num. AAA287554

• Gene Names: Mark4; C79806; Markl1; 2410090P21Rik
• Reactivity: Mouse
• Synonyms: MAP/microtubule affinity-regulating kinase 4 (MARK4); Mouse MAP/microtubule affinity-regulating kinase 4 (MARK4) ELISA Kit; FLJ90097; KIAA1860; MARKL1; Nbla00650; MAP/microtubule affinity-regulating kinase like 1; MARK4 serine/threonine protein kinase; putative protein product of Nbla00650; MARK4 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Mouse MARK4. No significant cross-reactivity or interference between Mouse MARK4 and analogues was observed.
• Sequence Length: 752

• Assay Type: Quantitive Sandwich
• Samples: Serum, Plasma, Other Biological Fluids
• Detection Wavelength: 450 nm
• Intra-assay Precision (Precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
• Inter-assay Precision (Precision between assays): Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
• CV (%): = SD/meanX100
• Intra-Assay: CV
• Inter-Assay: CV
• Preparation and Storage: Store at 2-8 degree C.

Related Product Information for MARK4 elisa kit

Description: This assay employs a two-site sandwich ELISA to quantitate MARK4 in samples. An antibody specific for MARK4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MARK4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MARK4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MARK4 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Overview: Sequence analysis predicted that the 752-amino acid MARKL1 protein, 62% identical to MARK3, contains an N-terminal serine/threonine kinase domain, a central ubiquitin-associated domain, and a C-terminal KA1 -associated kinase domain. A smaller transcript, MARKL1S, lacking exon 16 and the KA1-associated kinase domain in the 688-amino acid protein, was also detected exclusively in brain. RT-PCR analysis detected upregulated expression in nearly all clinical hepatocellular carcinoma cells. Northern blot analysis revealed ubiquitous expression of a 3.6-kb transcript, with highest expression in testis. Immunofluorescence microscopy demonstrated homogeneous cytoplasmic expression. Colony-forming assays showed that MARKL1 antisense reduced the growth of SNU475 cells.

NCBI and Uniprot Product Information

• NCBI GI #: 26986591
• NCBI GeneID: 232944
• NCBI Accession #: NP_758483.1
• NCBI GenBank Nucleotide #: NM_172279.1
• UniProt Accession #: Q8CIP4; Q80T81
• Molecular Weight: 75,415 Da
• NCBI Official Full Name: MAP/microtubule affinity-regulating kinase 4
• NCBI Official Synonym Full Names: MAP/microtubule affinity regulating kinase 4
• NCBI Official Symbol: Mark4
• NCBI Official Synonym Symbols: C79806; Markl1; 2410090P21Rik
• NCBI Protein Information: MAP/microtubule affinity-regulating kinase 4
• UniProt Protein Name: MAP/microtubule affinity-regulating kinase 4
• Protein Family: MAP/microtubule affinity-regulating kinase
• UniProt Gene Name: Mark4
• UniProt Synonym Gene Names: Kiaa1860

Uniprot Description

Serine/threonine-protein kinase (). Phosphorylates the microtubule-associated protein MAPT (). Also phosphorylates the microtubule-associated proteins MAP2 and MAP4 (). Involved in regulation of the microtubule network, causing reorganization of microtubules into bundles (). Required for the initiation of axoneme extension during cilium assembly (). Regulates the centrosomal location of ODF2 and phosphorylates ODF2 in vitro (). Plays a role in cell cycle progression, specifically in the G1/S checkpoint (). Reduces neuronal cell survival (). Plays a role in energy homeostasis by regulating satiety and metabolic rate (PubMed:22992738). Promotes adipogenesis by activating JNK1 and inhibiting the p38MAPK pathway, and triggers apoptosis by activating the JNK1 pathway (PubMed:24989893). Phosphorylates mTORC1 complex member RPTOR and acts as a negative regulator of the mTORC1 complex, probably due to disruption of the interaction between phosphorylated RPTOR and the RRAGA/RRAGC heterodimer which is required for mTORC1 activation ().