Human Mitochondrial import inner membrane translocase subunit Tim22 (TIMM22) ELISA Kit | TIMM22 elisa kit

Human Mitochondrial import inner membrane translocase subunit Tim22 (TIMM22) ELISA Kit

Cat. Num. AAA287433

• Gene Names: TIMM22; TEX4; TIM22
• Reactivity: Human
• Synonyms: Mitochondrial import inner membrane translocase subunit Tim22 (TIMM22); Human Mitochondrial import inner membrane translocase subunit Tim22 (TIMM22) ELISA Kit; TEX4; TIM22; putative membrane protein; testis-expressed sequence 4; translocase of inner mitochondrial membrane 22 homolog; TIMM22 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Human TIMM22. No significant cross-reactivity or interference between Human TIMM22 and analogues was observed.
• Sequence Length: 194

• Assay Type: Quantitive Sandwich
• Samples: Serum, Plasma, Other Biological Fluids
• Detection Wavelength: 450 nm
• Intra-assay Precision (Precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
• Inter-assay Precision (Precision between assays): Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
• CV (%): = SD/meanX100
• Intra-Assay: CV
• Inter-Assay: CV
• Preparation and Storage: Store at 2-8 degree C.

Related Product Information for TIMM22 elisa kit

This assay employs a two-site sandwich ELISA to quantitate TIMM22 in samples. An antibody specific for TIMM22 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TIMM22 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TIMM22 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TIMM22 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 56606061
• NCBI GeneID: 29928
• NCBI Accession #: NP_037469.2
• NCBI GenBank Nucleotide #: NM_013337.3
• UniProt Accession #: Q9Y584; Q9NWI8
• Molecular Weight: 20,031 Da
• NCBI Official Full Name: mitochondrial import inner membrane translocase subunit Tim22
• NCBI Official Synonym Full Names: translocase of inner mitochondrial membrane 22
• NCBI Official Symbol: TIMM22
• NCBI Official Synonym Symbols: TEX4; TIM22
• NCBI Protein Information: mitochondrial import inner membrane translocase subunit Tim22
• UniProt Protein Name: Mitochondrial import inner membrane translocase subunit Tim22
• Protein Family: Mitochondrial import inner membrane translocase
• UniProt Gene Name: TIMM22
• UniProt Synonym Gene Names: TEX4; TIM22

NCBI Description

Multipass transmembrane proteins are brought into mitochondria and inserted into the mitochondrial inner membrane by way of the TIM22 complex. This complex has six subunits and is a twin-pore translocase. The protein encoded by this gene is a subunit of TIM22 and represents the voltage-activated and signal-gated channel. [provided by RefSeq, Jul 2016]

Uniprot Description

Essential core component of the TIM22 complex, a complex that mediates the import and insertion of multi-pass transmembrane proteins into the mitochondrial inner membrane. In the TIM22 complex, it constitutes the voltage-activated and signal-gated channel. Forms a twin-pore translocase that uses the membrane potential as external driving force in 2 voltage-dependent steps ().