Human Transmembrane protein 132A (TMEM132A) ELISA Kit | TMEM132A elisa kit
Human Transmembrane protein 132A (TMEM132A) ELISA Kit
Gene Names
TMEM132A; GBP; HSPA5BP1
Reactivity
Human
Synonyms
Transmembrane protein 132A (TMEM132A); Human Transmembrane protein 132A (TMEM132A) ELISA Kit; DKFZp547E212; FLJ20539; GBP; HSPA5BP1; MGC138669; GRP78-binding protein; glucose-regulated protein; 78kDa; heat shock 70kDa protein 5 (glucose-regulated protein; 78kDa) binding protein 1; heat shock 70; TMEM132A elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of Human TMEM132A. No significant cross-reactivity or interference between Human TMEM132A and analogues was observed.
Sequence Length
1024
Samples
Serum, plasma and other biological fluids
Assay Type
Quantitative Sandwich
Detection Range
78.1 pg/mL - 5000 pg/mL
Sensitivity
39 pg/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Intra-Assay: CV<8%
Inter-assay Precision
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in forty separate assays to assess inter-assay precision. CV (%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
Store at 2-8 degree C.
Related Product Information for TMEM132A elisa kit
Intended Uses: For the quantitative detection of Human Transmembrane protein 132A (TMEM132A) concentration in serum, plasma and other biological fluids.
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate TMEM132A in Human serum, plasma. An antibody specific for TMEM132A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TMEM132A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TMEM132A is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TMEM132A bound in the initial step. The color development is stopped and the intensity of the color is measured.
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate TMEM132A in Human serum, plasma. An antibody specific for TMEM132A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TMEM132A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TMEM132A is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TMEM132A bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
83,098 Da
NCBI Official Full Name
transmembrane protein 132A isoform a
NCBI Official Synonym Full Names
transmembrane protein 132A
NCBI Official Symbol
TMEM132A
NCBI Official Synonym Symbols
GBP; HSPA5BP1
NCBI Protein Information
transmembrane protein 132A
UniProt Protein Name
Transmembrane protein 132A
Protein Family
UniProt Gene Name
TMEM132A
UniProt Synonym Gene Names
HSPA5BP1; KIAA1583
NCBI Description
This gene encodes a protein that is highly similar to the rat Grp78-binding protein (GBP). Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008]
Uniprot Description
May play a role in embryonic and postnatal development of the brain. Increased resistance to cell death induced by serum starvation in cultured cells. Regulates cAMP-induced GFAP gene expression via STAT3 phosphorylation ().
Research Articles on TMEM132A
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Product Notes
The Human TMEM132A tmem132a (Catalog #AAA287419) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA287419 ELISA Kit recognizes Human TMEM132A. It is sometimes possible for the material contained within the vial of "Transmembrane protein 132A (TMEM132A), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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