Human H/ACA ribonucleoprotein complex subunit 2 (NHP2) ELISA Kit | NHP2 elisa kit

Human H/ACA ribonucleoprotein complex subunit 2 (NHP2) ELISA Kit

Cat. Num. AAA286899

• Gene Names: NHP2; DKCB2; NHP2P; NOLA2
• Reactivity: Human
• Synonyms: H/ACA ribonucleoprotein complex subunit 2 (NHP2); Human H/ACA ribonucleoprotein complex subunit 2 (NHP2) ELISA Kit; NHP2P; NOLA2; NHP2-like protein; nucleolar protein family A; member 2; member 2 (H/ACA small nucleolar RNPs); NHP2 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Human NHP2. No significant cross-reactivity or interference between Human NHP2 and analogues was observed.
• Sequence Length: 90

• Assay Type: Quantitive Sandwich
• Samples: Serum, Plasma, Other Biological Fluids
• Detection Wavelength: 450 nm
• Intra-assay Precision (Precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
• Inter-assay Precision (Precision between assays): Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
• CV (%): = SD/meanX100
• Intra-Assay: CV
• Inter-Assay: CV
• Preparation and Storage: Store at 2-8 degree C.

Related Product Information for NHP2 elisa kit

This assay employs a two-site sandwich ELISA to quantitate NHP2 in samples. An antibody specific for NHP2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any NHP2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NHP2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NHP2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 77812674
• NCBI GeneID: 55651
• NCBI Accession #: NP_001030005.1
• NCBI GenBank Nucleotide #: NM_001034833.1
• UniProt Accession #: Q9NX24; Q9P095; A6NKY8
• Molecular Weight: 17,201 Da
• NCBI Official Full Name: H/ACA ribonucleoprotein complex subunit 2 isoform b
• NCBI Official Synonym Full Names: NHP2 ribonucleoprotein
• NCBI Official Symbol: NHP2
• NCBI Official Synonym Symbols: DKCB2; NHP2P; NOLA2
• NCBI Protein Information: H/ACA ribonucleoprotein complex subunit 2
• UniProt Protein Name: H/ACA ribonucleoprotein complex subunit 2
• Protein Family: H/ACA ribonucleoprotein complex
• UniProt Gene Name: NHP2
• UniProt Synonym Gene Names: NOLA2

NCBI Description

This gene is a member of the H/ACA snoRNPs (small nucleolar ribonucleoproteins) gene family. snoRNPs are involved in various aspects of rRNA processing and modification and have been classified into two families: C/D and H/ACA. The H/ACA snoRNPs also include the DKC1, NOLA1 and NOLA3 proteins. These four H/ACA snoRNP proteins localize to the dense fibrillar components of nucleoli and to coiled (Cajal) bodies in the nucleus. Both 18S rRNA production and rRNA pseudouridylation are impaired if any one of the four proteins is depleted. The four H/ACA snoRNP proteins are also components of the telomerase complex. This gene encodes a protein related to Saccharomyces cerevisiae Nhp2p. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2008]

Uniprot Description

Required for ribosome biogenesis and telomere maintenance. Part of the H/ACA small nucleolar ribonucleoprotein (H/ACA snoRNP) complex, which catalyzes pseudouridylation of rRNA. This involves the isomerization of uridine such that the ribose is subsequently attached to C5, instead of the normal N1. Each rRNA can contain up to 100 pseudouridine ("psi") residues, which may serve to stabilize the conformation of rRNAs. May also be required for correct processing or intranuclear trafficking of TERC, the RNA component of the telomerase reverse transcriptase (TERT) holoenzyme.