Porcine Zonadhesin (ZAN) ELISA Kit | ZAN elisa kit
Pig Zonadhesin (ZAN) ELISA Kit
Reactivity
Porcine
Synonyms
Zonadhesin (ZAN); Pig Zonadhesin (ZAN) ELISA Kit; OTTHUMP00000211358; OTTHUMP00000211363; ZAN elisa kit
Reactivity
Porcine
Specificity
This assay has high sensitivity and excellent specificity for detection of Pig ZAN. No significant cross-reactivity or interference between Pig ZAN and analogues was observed.
Sequence Length
2476
Assay Type
Quantitive Sandwich
Samples
Serum, Plasma, Other Biological Fluids
Detection Wavelength
450 nm
Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%)
= SD/meanX100
Intra-Assay
CV
Inter-Assay
CV
Preparation and Storage
Store at 2-8 degree C.
Related Product Information for ZAN elisa kit
Description: This assay employs a two-site sandwich ELISA to quantitate ZAN in samples. An antibody specific for ZAN has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ZAN present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ZAN is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ZAN bound in the initial step. The color development is stopped and the intensity of the color is measured.
Overview: Zonadhesin (ZAN) is a sperm membrane protein that binds the zona pellucida of the egg in a species-specific manner. Once bound, the acrosomal reaction is triggered, involving exocytosis of the acrosome, creating an acrosomal shroud. Next, the sperm penetrates the egg zona pellucida, enters the perivitelline space between the zona pellucida and the plasma membrane, and then fuses with the plasma membrane. The zonadhesin protein, encoded by the ZAN gene, localizes to the anterior part of the sperm head and acts as a receptor to the zona pellucida matrix of the egg. Like other male reproductive proteins located in the acrosome or sperm head and proposed to be involved in species-specific binding to eggs, ZAN possesses high levels of divergence between closely related species.
Overview: Zonadhesin (ZAN) is a sperm membrane protein that binds the zona pellucida of the egg in a species-specific manner. Once bound, the acrosomal reaction is triggered, involving exocytosis of the acrosome, creating an acrosomal shroud. Next, the sperm penetrates the egg zona pellucida, enters the perivitelline space between the zona pellucida and the plasma membrane, and then fuses with the plasma membrane. The zonadhesin protein, encoded by the ZAN gene, localizes to the anterior part of the sperm head and acts as a receptor to the zona pellucida matrix of the egg. Like other male reproductive proteins located in the acrosome or sperm head and proposed to be involved in species-specific binding to eggs, ZAN possesses high levels of divergence between closely related species.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
270,365 Da
NCBI Official Full Name
zonadhesin
NCBI Official Symbol
ZAN
NCBI Protein Information
zonadhesin
UniProt Protein Name
Zonadhesin
Protein Family
UniProt Gene Name
ZAN
Uniprot Description
Binds in a species-specific manner to the zona pellucida of the egg. May be involved in gamete recognition and/or signaling.
Similar Products
Product Notes
The Porcine ZAN zan (Catalog #AAA286608) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA286608 ELISA Kit recognizes Porcine ZAN. It is sometimes possible for the material contained within the vial of "Zonadhesin (ZAN), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
