Rabbit anti-Human ATP5S Polyclonal Antibody | anti-ATP5S antibody

ATP5S, NT (ATP5S, ATPW, ATP synthase subunit s, mitochondrial, ATP synthase-coupling factor B, Mitochondrial ATP synthase regulatory component factor B) (APC)

Cat. Num. AAA6276602

• Gene Names: ATP5S; FB; ATPW; HSU79253
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: ATP5S; Polyclonal Antibody; NT (ATP5S; ATPW; ATP synthase subunit s; mitochondrial; ATP synthase-coupling factor B; Mitochondrial ATP synthase regulatory component factor B) (APC); mitochondrial ATP synthase regulatory component factor B; anti-ATP5S antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-ATP5S antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: ATP5S antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 36-64 amino acids from the N-terminal region of human ATP5S.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(ATP5S Antibody (N-term) western blot analysis in MDA-MB453 cell line lysates (35ug/lane).This demonstrates the ATP5S antibody detected the ATP5S protein (arrow).)

Product Categories/Family for anti-ATP5S antibody

Antibodies; Mitochondrial Proteins

References

Ma, J., et al. Atherosclerosis 191(1):63-72(2007). Cross, R.L. Nature 427(6973):407-408(2004). Oster, G., et al. Trends Cell Biol. 13(3):114-121(2003). Leyva, J.A., et al. Mol. Membr. Biol. 20(1):27-33(2003). Belogrudov, G.I., et al. J. Biol. Chem. 277(8):6097-6103(2002).

NCBI and Uniprot Product Information

• NCBI GI #: 51558774
• NCBI GeneID: 27109
• NCBI Accession #: NP_001003803.1
• NCBI GenBank Nucleotide #: NM_001003803.2
• UniProt Accession #: Q99766; Q8WWX3; Q96F77; A8K1U3; D9N156
• Molecular Weight: 24,866 Da
• NCBI Official Full Name: ATP synthase subunit s, mitochondrial isoform a
• NCBI Official Synonym Full Names: ATP synthase, H+ transporting, mitochondrial Fo complex, subunit s (factor B)
• NCBI Official Symbol: ATP5S
• NCBI Official Synonym Symbols: FB; ATPW; HSU79253
• NCBI Protein Information: ATP synthase subunit s, mitochondrial; ATP synthase-coupling factor B; ATP synthase coupling factor B-like 1; ATP synthase coupling factor B, mitochondrial; mitochondrial ATP synthase regulatory component factor B; ATP synthase, H+ transporting, mitochond
• UniProt Protein Name: ATP synthase subunit s, mitochondrial
• Protein Family: ATP synthase
• UniProt Gene Name: ATP5S
• UniProt Synonym Gene Names: ATPW; FB
• UniProt Entry Name: ATP5S_HUMAN

NCBI Description

This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. This gene encodes the subunit s, also known as factor B, of the proton channel. This subunit is necessary for the energy transduction activity of the ATP synthase complexes. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]

Uniprot Description

ATP5S: Involved in regulation of mitochondrial membrane ATP synthase. Necessary for H(+) conduction of ATP synthase. Facilitates energy-driven catalysis of ATP synthesis by blocking a proton leak through an alternative proton exit pathway. Belongs to the ATP synthase subunit s family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Mitochondrial

Chromosomal Location of Human Ortholog: 14q21.3

Cellular Component: proton-transporting ATP synthase complex, coupling factor F(o); mitochondrial inner membrane

Molecular Function: metal ion binding; hydrogen ion transmembrane transporter activity

Biological Process: cellular metabolic process; proton transport; mitochondrial ATP synthesis coupled proton transport