Chicken Endothelial differentiation-related factor 1 ELISA Kit | EDF1 elisa kit
Chicken Endothelial differentiation-related factor 1 (EDF1) ELISA Kit
Reactivity
Chicken
Synonyms
Endothelial differentiation-related factor 1; Chicken Endothelial differentiation-related factor 1 (EDF1) ELISA Kit; RP11-216L13.1; EDF-1; MBF1; MGC9058; multiprotein bridging factor 1|multiprotein bridging factor-1; EDF1 elisa kit
Reactivity
Chicken
Specificity
This assay has high sensitivity and excellent specificity for detection of Chicken EDF1. No significant cross-reactivity or interference between Chicken EDF1 and analogues was observed.
Sequence Length
148
Samples
Serum, Plasma, Other biological fluids
Assay Type
Sandwich
Sample Volume
1-200 uL
Precision
Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
Detection Wavelength
450 nm
Preparation and Storage
Store at 2-8 degree C.
Related Product Information for EDF1 elisa kit
Endothelial differentiation-related factor 1 is a protein that may regulate endothelial cell differentiation. It has been postulated that the protein functions as a bridging molecule that interconnects regulatory proteins and the basal transcriptional machinery, thereby modulating the transcription of genes involved in endothelial differentiation.
This protein has also been found to act as a transcriptional coactivator by interconnecting the general transcription factor TATA element-binding protein (TBP) and gene-specific activators. Two alternatively spliced transcripts which encode distinct proteins have been found for this gene.Expressed in brain, liver, lung, kidney and heart (at protein level). Ubiquitously expressed. More abundant in heart, pancreas, liver, intestine and adipose tissues.
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate EDF1 in samples. An antibody specific for EDF1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyEDF1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for EDF1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of EDF1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
This protein has also been found to act as a transcriptional coactivator by interconnecting the general transcription factor TATA element-binding protein (TBP) and gene-specific activators. Two alternatively spliced transcripts which encode distinct proteins have been found for this gene.Expressed in brain, liver, lung, kidney and heart (at protein level). Ubiquitously expressed. More abundant in heart, pancreas, liver, intestine and adipose tissues.
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate EDF1 in samples. An antibody specific for EDF1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyEDF1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for EDF1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of EDF1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
16,364 Da
NCBI Official Full Name
endothelial differentiation-related factor 1 homolog
NCBI Official Synonym Full Names
endothelial differentiation related factor 1
NCBI Official Symbol
EDF1
NCBI Protein Information
endothelial differentiation-related factor 1 homolog
UniProt Protein Name
Endothelial differentiation-related factor 1 homolog
Protein Family
UniProt Gene Name
EDF1
UniProt Synonym Gene Names
EDF-1
Uniprot Description
Probable transcriptional coactivator.
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Product Notes
The Chicken EDF1 edf1 (Catalog #AAA285512) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA285512 ELISA Kit recognizes Chicken EDF1. It is sometimes possible for the material contained within the vial of "Endothelial differentiation-related factor 1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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