Human Leukocyte esterase ELISA Kit | LE elisa kit
Human Leukocyte esterase (LE) ELISA Kit
Gene Names
LDAH; hLDAH; C2orf43
Reactivity
Human
Synonyms
Leukocyte esterase; Human Leukocyte esterase (LE) ELISA Kit; LE elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of Human LE. No significant cross-reactivity or interference between Human LE and analogues was observed.
Sequence Length
195
Samples
Serum, plasma and other biological fluids
Assay Type
Quantitative Sandwich
Detection Range
31.25 pg/mL - 2000 pg/mL
Sensitivity
15.6 pg/mL (mean of 6 independent assays)
Intra-assay Precision
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Intra-Assay: CV<8%
Inter-assay Precision
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in forty separate assays to assess inter-assay precision. CV (%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
Store at 2-8 degree C.
Related Product Information for LE elisa kit
Intended Uses: For the quantitative detection of Human Leukocyte esterase (LE) concentration in serum, plasma and other biological fluids.
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate LE in Human serum, plasma. An antibody specific for LE has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any LE present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LE is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LE bound in the initial step. The color development is stopped and the intensity of the color is measured.
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate LE in Human serum, plasma. An antibody specific for LE has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any LE present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LE is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LE bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
22,663 Da
NCBI Official Full Name
lipid droplet-associated hydrolase isoform d
NCBI Official Synonym Full Names
lipid droplet associated hydrolase
NCBI Official Symbol
LDAH
NCBI Official Synonym Symbols
hLDAH; C2orf43
NCBI Protein Information
lipid droplet-associated hydrolase
UniProt Protein Name
Lipid droplet-associated hydrolase
UniProt Gene Name
LDAH
UniProt Synonym Gene Names
hLDAH
UniProt Entry Name
LDAH_HUMAN
Uniprot Description
LDAH: Belongs to the UPF0554 family. 2 isoforms of the human protein are produced by alternative splicing.
Chromosomal Location of Human Ortholog: 2p24.1
Cellular Component: endoplasmic reticulum; lipid particle
Molecular Function: lipase activity
Biological Process: lipid catabolic process; sequestering of lipid
Research Articles on LE
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Product Notes
The Human LE ldah (Catalog #AAA285418) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA285418 ELISA Kit recognizes Human LE. It is sometimes possible for the material contained within the vial of "Leukocyte esterase, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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