Human Roquin ELISA Kit | RC3H1 elisa kit

Human Roquin (RC3H1) ELISA Kit

Cat. Num. AAA285411

• Gene Names: RC3H1; RNF198; ROQUIN
• Reactivity: Human
• Synonyms: Roquin; Human Roquin (RC3H1) ELISA Kit; RP5-1198E17.5; KIAA2025; RNF198; ROQUIN; roquin; RC3H1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Human RC3H1. No significant cross-reactivity or interference between Human RC3H1 and analogues was observed.
• Sequence Length: 1111

• Samples: Serum, Plasma, Other biological fluids
• Assay Type: Sandwich
• Sample Volume: 1-200 uL
• Precision: Intra-assay Precision (Precision within an assay); Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.; Inter-assay Precision (Precision between assays); Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.; CV (%) = SD/meanX100; Intra-Assay: CV; Inter-Assay: CV
• Detection Wavelength: 450 nm
• Preparation and Storage: Store at 2-8 degree C.

Related Product Information for RC3H1 elisa kit

The roquin protein is distinguished by the presence of a CCCH zinc finger found in RNA-binding proteins, and localization to cytosolic RNA granules implicated in regulating mRNA translation and stability.Roquin mRNA transcripts were found ubiquitously. The roquin protein is predicted to be a 1,130-residue intracellular protein highly conserved across its full length in mammals to invertebrates. The protein has an amino-terminal RING-1 zinc finger at residues 14-53 that conforms perfectly to the consensus for the E3 ubiquitin ligase family of proteins. Carboxy-terminal to the RING finger (residues 131-360) is a highly conserved novel protein domain (ROQ domain). A zinc finger domain of the C-X8-C-X5-C-X3-H type (CCCH type) occurs in the middle of roquin (residues 419-438).

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate RC3H1 in samples. An antibody specific for RC3H1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyRC3H1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RC3H1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RC3H1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 664806080
• NCBI GeneID: 149041
• NCBI Accession #: NP_001287779.1
• NCBI GenBank Nucleotide #: NM_001300850.1
• UniProt Accession #: Q5TC82; Q5W180; Q5W181; Q8IVE6; Q8N9V1; B3KVK1
• Molecular Weight: 124,842 Da
• NCBI Official Full Name: roquin-1 isoform a
• NCBI Official Synonym Full Names: ring finger and CCCH-type domains 1
• NCBI Official Symbol: RC3H1
• NCBI Official Synonym Symbols: RNF198; ROQUIN
• NCBI Protein Information: roquin-1
• UniProt Protein Name: Roquin-1
• Protein Family: Roquin
• UniProt Gene Name: RC3H1
• UniProt Synonym Gene Names: KIAA2025; RNF198; Roquin

NCBI Description

This gene encodes a protein containing RING-type and C3H1-type zinc finger motifs. The encoded protein recognizes and binds to a constitutive decay element (CDE) in the 3' UTR of mRNAs, leading to mRNA deadenylation and degradation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014]

Uniprot Description

RC3H1: Represses ICOS function, an essential costimulatory receptor for follicular T-cells. May act as an ubiquitin ligase that regulates ubiquitin-dependent control of AU-rich mRNAs by AUF1/HNRPD and HUR/ELAVL1. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: RNA-binding; Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 1q25.1

Cellular Component: stress granule

Molecular Function: metal ion binding; mRNA 3'-UTR binding; RNA binding

Biological Process: B cell homeostasis; cytoplasmic mRNA processing body assembly; lymph node development; mRNA catabolic process, deadenylation-dependent decay; negative regulation of activated T cell proliferation; negative regulation of B cell proliferation; negative regulation of germinal center formation; negative regulation of T-helper cell differentiation; protein ubiquitination; regulation of germinal center formation; regulation of mRNA stability; regulation of T cell receptor signaling pathway; spleen development; T cell homeostasis; T cell proliferation