Chicken E3 ubiquitin-protein ligase BRE1A ELISA Kit | RNF20 elisa kit
Chicken E3 ubiquitin-protein ligase BRE1A (RNF20) ELISA Kit
Reactivity
Chicken
Synonyms
E3 ubiquitin-protein ligase BRE1A; Chicken E3 ubiquitin-protein ligase BRE1A (RNF20) ELISA Kit; BRE1; BRE1A; FLJ11189; FLJ20382; KIAA2779; MGC129667; MGC129668; hBRE1; BRE1 E3 ubiquitin ligase homolog|homolog of S. cerevisiae BRE1; RNF20 elisa kit
Reactivity
Chicken
Specificity
This assay has high sensitivity and excellent specificity for detection of Chicken RNF20. No significant cross-reactivity or interference between Chicken RNF20 and analogues was observed.
Sequence Length
975
Samples
Serum, Plasma, Other biological fluids
Assay Type
Sandwich
Sample Volume
1-200 uL
Precision
Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
Detection Wavelength
450 nm
Preparation and Storage
Store at 2-8 degree C.
Related Product Information for RNF20 elisa kit
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate RNF20 in samples. An antibody specific for RNF20 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyRNF20 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RNF20 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RNF20 bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
114,808 Da
NCBI Official Full Name
E3 ubiquitin-protein ligase BRE1A
NCBI Official Synonym Full Names
ring finger protein 20
NCBI Official Symbol
RNF20
NCBI Protein Information
E3 ubiquitin-protein ligase BRE1A
UniProt Protein Name
E3 ubiquitin-protein ligase BRE1A
Protein Family
UniProt Gene Name
RNF20
UniProt Synonym Gene Names
BRE1A; BRE1-A
Uniprot Description
Component of the RNF20/40 E3 ubiquitin-protein ligase complex that mediates monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1). H2BK120ub1 gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation (H3K4me and H3K79me, respectively).
Research Articles on RNF20
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Product Notes
The Chicken RNF20 rnf20 (Catalog #AAA283759) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA283759 ELISA Kit recognizes Chicken RNF20. It is sometimes possible for the material contained within the vial of "E3 ubiquitin-protein ligase BRE1A, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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