Human Putative pre-mRNA-splicing factor ATP-dependent RNA helicase DHX15 ELISA Kit | DHX15 elisa kit
Human Putative pre-mRNA-splicing factor ATP-dependent RNA helicase DHX15 (DHX15) ELISA Kit
Gene Names
DHX15; DBP1; HRH2; DDX15; PRP43; PRPF43; PrPp43p
Reactivity
Human
Synonyms
Putative pre-mRNA-splicing factor ATP-dependent RNA helicase DHX15; Human Putative pre-mRNA-splicing factor ATP-dependent RNA helicase DHX15 (DHX15) ELISA Kit; DHX15 elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of Human DHX15. No significant cross-reactivity or interference between Human DHX15 and analogues was observed.
Sequence Length
795
Samples
Serum, plasma and other biological fluids
Assay Type
Quantitative Sandwich
Detection Range
0.31 ng/mL - 20 ng/mL
Sensitivity
0.1 ng/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Intra-Assay: CV<8%
Inter-assay Precision
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in forty separate assays to assess inter-assay precision. CV (%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
Store at 2-8 degree C.
Related Product Information for DHX15 elisa kit
Intended Uses: For the quantitative detection of Human Putative pre-mRNA-splicing factor ATP-dependent RNA helicase DHX15 (DHX15) concentration in serum, plasma and other biological fluids.
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate DHX15 in Human serum, plasma. An antibody specific for DHX15 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any DHX15 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DHX15 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DHX15 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate DHX15 in Human serum, plasma. An antibody specific for DHX15 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any DHX15 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DHX15 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DHX15 bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
90,933 Da
NCBI Official Full Name
pre-mRNA-splicing factor ATP-dependent RNA helicase DHX15
NCBI Official Synonym Full Names
DEAH-box helicase 15
NCBI Official Symbol
DHX15
NCBI Official Synonym Symbols
DBP1; HRH2; DDX15; PRP43; PRPF43; PrPp43p
NCBI Protein Information
pre-mRNA-splicing factor ATP-dependent RNA helicase DHX15
UniProt Protein Name
Pre-mRNA-splicing factor ATP-dependent RNA helicase DHX15
Protein Family
UniProt Gene Name
DHX15
UniProt Synonym Gene Names
DBP1; DDX15
NCBI Description
The protein encoded by this gene is a putative ATP-dependent RNA helicase implicated in pre-mRNA splicing. [provided by RefSeq, Jul 2008]
Uniprot Description
DDX15: Pre-mRNA processing factor involved in disassembly of spliceosomes after the release of mature mRNA. Belongs to the DEAD box helicase family. DEAH subfamily. DDX15/PRP43 sub-subfamily.
Protein type: EC 3.6.4.13; Helicase; Nucleolus; RNA splicing; RNA-binding; Spliceosome
Chromosomal Location of Human Ortholog: 4p15.2
Cellular Component: cytoplasm; nuclear speck; nucleolus; nucleoplasm; nucleus; U12-type spliceosomal complex; U2-type post-mRNA release spliceosomal complex
Molecular Function: ATP binding; ATP-dependent RNA helicase activity; double-stranded RNA binding; protein binding; RNA binding; RNA helicase activity
Biological Process: mRNA processing; mRNA splicing, via spliceosome; response to alkaloid; response to toxin; RNA splicing
Research Articles on DHX15
1. The crystal structure of human DHX15 reveals a domain organization of the mammalian DEAH/RHA family.
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Product Notes
The Human DHX15 dhx15 (Catalog #AAA283340) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA283340 ELISA Kit recognizes Human DHX15. It is sometimes possible for the material contained within the vial of "Putative pre-mRNA-splicing factor ATP-dependent RNA helicase DHX15, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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