Horse Regulator of G-protein signaling 1 ELISA Kit | RGS1 elisa kit

Horse Regulator of G-protein signaling 1 (RGS1) ELISA Kit

Cat. Num. AAA282873

• Reactivity: Horse
• Synonyms: Regulator of G-protein signaling 1; Horse Regulator of G-protein signaling 1 (RGS1) ELISA Kit; RGS1 elisa kit

• Reactivity: Horse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Horse RGS1. No significant cross-reactivity or interference between Horse RGS1 and analogues was observed.
• Sequence Length: 209

• Samples: Serum, Plasma, Other biological fluids
• Assay Type: Sandwich
• Sample Volume: 1-200 uL
• Precision: Intra-assay Precision (Precision within an assay); Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.; Inter-assay Precision (Precision between assays); Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.; CV (%) = SD/meanX100; Intra-Assay: CV; Inter-Assay: CV
• Detection Wavelength: 450 nm
• Preparation and Storage: Store at 2-8 degree C.

Related Product Information for RGS1 elisa kit

Regulator of G-protein signaling 1 is a member of the regulator of G-protein signalling family. This protein is located on the cytosolic side of the plasma membrane and contains a conserved, 120 amino acid motif called the RGS domain. The protein attenuates the signalling activity of G-proteins by binding to activated, GTP-bound G alpha subunits and acting as a GTPase activating protein (GAP), increasing the rate of conversion of the GTP to GDP. This hydrolysis allows the G alpha subunits to bind G beta/gamma subunit heterodimers, forming inactive G-protein heterotrimers, thereby terminating the signal.B-cell specific. Expression is relatively low in B-cells and chronic lymphocytic leukemia B-cells; however, in other types of malignant B-cell such as non-Hodgkin's lymphoma and hairy cell leukemia, expression is constitutively high.

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate RGS1 in samples. An antibody specific for RGS1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyRGS1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RGS1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RGS1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 126352324
• NCBI GeneID: 100034151
• NCBI Accession #: NP_001075408.1
• NCBI GenBank Nucleotide #: NM_001081939.1
• UniProt Accession #: Q6RG78
• Molecular Weight: 22,544 Da
• NCBI Official Full Name: regulator of G-protein signaling 1
• NCBI Official Synonym Full Names: regulator of G protein signaling 1
• NCBI Official Symbol: RGS1
• NCBI Protein Information: regulator of G-protein signaling 1
• UniProt Protein Name: Regulator of G-protein signaling 1
• Protein Family: Regulator of G-protein signaling
• UniProt Gene Name: RGS1
• UniProt Synonym Gene Names: RGS1

Uniprot Description

Regulates G protein-coupled receptor signaling cascades, including signaling downstream of the N-formylpeptide chemoattractant receptors and leukotriene receptors. Inhibits B cell chemotaxis toward CXCL12 (). Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits, thereby driving them into their inactive GDP-bound form ().