Mouse Zinc finger protein 426 ELISA Kit | ZNF426 elisa kit
Mouse Zinc finger protein 426 (ZNF426) ELISA Kit
Gene Names
Zfp426; KRAB1; Zfo61; Znf426; Zfp68-rs1; mKIAA4237; 2900057C04Rik
Reactivity
Mouse
Synonyms
Zinc finger protein 426; Mouse Zinc finger protein 426 (ZNF426) ELISA Kit; ZNF426 elisa kit
Reactivity
Mouse
Specificity
This assay has high sensitivity and excellent specificity for detection of Mouse ZNF426. No significant cross-reactivity or interference between Mouse ZNF426 and analogues was observed.
Sequence Length
554
Samples
Serum, Plasma, Other biological fluids
Assay Type
Sandwich
Sample Volume
1-200 uL
Precision
Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
Detection Wavelength
450 nm
Preparation and Storage
Store at 2-8 degree C.
Related Product Information for ZNF426 elisa kit
Zinc finger proteins contain DNA-binding domains and have a wide variety of functions, most of which encompass some form of transcriptional activation or repression. The majority of zinc finger proteins contain a Krüpel-type DNA binding domain and a KRAB domain, which is thought to interact with KAP1, thereby recruiting histone modifying proteins.
ZNF426 (zinc finger protein 426), also known as MGC2663, is a 554 amino acid protein that is thought to be involved in transcriptional regulation. Localized to the nucleus, ZNF426 contains one KRAB domain and 12 C2H2-type zinc fingers through which it may convey DNA, RNA and protein binding capabilities. Specifically, ZNF426 may interact with the viral protein KSHV ORF 50 and, through this interaction, may activate viral gene transcription.
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate ZNF426 in samples. An antibody specific for ZNF426 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyZNF426 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ZNF426 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ZNF426 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ZNF426 (zinc finger protein 426), also known as MGC2663, is a 554 amino acid protein that is thought to be involved in transcriptional regulation. Localized to the nucleus, ZNF426 contains one KRAB domain and 12 C2H2-type zinc fingers through which it may convey DNA, RNA and protein binding capabilities. Specifically, ZNF426 may interact with the viral protein KSHV ORF 50 and, through this interaction, may activate viral gene transcription.
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate ZNF426 in samples. An antibody specific for ZNF426 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyZNF426 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ZNF426 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ZNF426 bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
63,020 Da
NCBI Official Full Name
zinc finger protein 426 isoform 1
NCBI Official Synonym Full Names
zinc finger protein 426
NCBI Official Symbol
Zfp426
NCBI Official Synonym Symbols
KRAB1; Zfo61; Znf426; Zfp68-rs1; mKIAA4237; 2900057C04Rik
NCBI Protein Information
zinc finger protein 426
UniProt Protein Name
Zinc finger protein 426
Protein Family
UniProt Gene Name
Znf426
Uniprot Description
ZNF426: May be involved in transcriptional regulation.
Protein type: C2H2-type zinc finger protein; DNA-binding; Transcription regulation
Chromosomal Location of Human Ortholog: 9 A3|9 7.55 cM
Cellular Component: intracellular; nucleus
Molecular Function: DNA binding; metal ion binding; nucleic acid binding
Biological Process: regulation of transcription, DNA-templated; transcription, DNA-dependent
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Product Notes
The Mouse ZNF426 znf426 (Catalog #AAA282808) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA282808 ELISA Kit recognizes Mouse ZNF426. It is sometimes possible for the material contained within the vial of "Zinc finger protein 426, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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