Mouse Apolipoprotein ELISA Kit | apo-A1 elisa kit
Mouse Apolipoprotein (apo-A1)ELISA Kit
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
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Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate E1 in samples. An antibody specific for E1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyE1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for E1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of E1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes apolipoprotein (apo-) A-II, which is the second most abundant protein of the high density lipoprotein particles. The protein is found in plasma as a monomer, homodimer, or heterodimer with apolipoprotein D. Defects in this gene may result in apolipoprotein A-II deficiency or hypercholesterolemia. [provided by RefSeq, Jul 2008]
Uniprot Description
APOA2: May stabilize HDL (high density lipoprotein) structure by its association with lipids, and affect the HDL metabolism. Belongs to the apolipoprotein A2 family.
Protein type: Lipid-binding; Secreted; Secreted, signal peptide; Endoplasmic reticulum
Chromosomal Location of Human Ortholog: 1q23.3
Cellular Component: chylomicron; endoplasmic reticulum lumen; early endosome; extracellular region; cytosol
Molecular Function: lipid transporter activity; protein binding; protein homodimerization activity; lipase inhibitor activity; protein heterodimerization activity; cholesterol transporter activity; cholesterol binding; phospholipid binding; phosphatidylcholine binding; high-density lipoprotein binding; lipid binding; apolipoprotein receptor binding
Biological Process: positive regulation of catalytic activity; phototransduction, visible light; protein folding; viral reproduction; negative regulation of cholesterol transport; response to glucocorticoid stimulus; regulation of protein stability; diacylglycerol catabolic process; positive regulation of lipid catabolic process; cellular lipid metabolic process; negative regulation of lipase activity; regulation of cholesterol absorption; phospholipid efflux; negative regulation of lipid catabolic process; response to glucose stimulus; retinoid metabolic process; response to drug; cholesterol metabolic process; organ regeneration; protein amino acid oxidation; phospholipid catabolic process; positive regulation of interleukin-8 biosynthetic process; cholesterol efflux; lipoprotein metabolic process; negative regulation of cytokine secretion during immune response; cholesterol homeostasis; triacylglycerol metabolic process; reverse cholesterol transport; response to estrogen stimulus; peptidyl-methionine modification; phosphatidylcholine biosynthetic process; acute inflammatory response
Disease: Hypercholesterolemia, Familial
Research Articles on apo-A1
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Product Notes
The Mouse apo-A1 apoa2 (Catalog #AAA282786) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA282786 ELISA Kit recognizes Mouse apo-A1. It is sometimes possible for the material contained within the vial of "Apolipoprotein, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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