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Pigeon Ubiquitin-fold modifier 1 ELISA Kit | UFM1 elisa kit

Pigeon Ubiquitin-fold modifier 1 (UFM1) ELISA Kit

Gene Names
UFM1; BM-002; C13orf20
Reactivity
Pigeon
Synonyms
Ubiquitin-fold modifier 1; Pigeon Ubiquitin-fold modifier 1 (UFM1) ELISA Kit; UFM1 elisa kit
Ordering
For Research Use Only!
Reactivity
Pigeon
Specificity
This assay has high sensitivity and excellent specificity for detection of Pig UFM1. No significant cross-reactivity or interference between Pig UFM1 and analogues was observed.
Sequence Length
85
Samples
Serum, Plasma, Other biological fluids
Assay Type
Sandwich
Sample Volume
1-200 uL
Precision
Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
Detection Wavelength
450 nm
Preparation and Storage
Store at 2-8 degree C.
Related Product Information for UFM1 elisa kit
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate UFM1 in samples. An antibody specific for UFM1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyUFM1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for UFM1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of UFM1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
10,960 Da
NCBI Official Full Name
ubiquitin-fold modifier 1 isoform 2
NCBI Official Synonym Full Names
ubiquitin fold modifier 1
NCBI Official Symbol
UFM1
NCBI Official Synonym Symbols
BM-002; C13orf20
NCBI Protein Information
ubiquitin-fold modifier 1
UniProt Protein Name
Ubiquitin-fold modifier 1
Protein Family
UniProt Gene Name
UFM1
UniProt Synonym Gene Names
C13orf20

NCBI Description

UFM1 is a ubiquitin-like protein that is conjugated to target proteins by E1-like activating enzyme UBA5 (UBE1DC1; MIM 610552) and E2-like conjugating enzyme UFC1 (MIM 610554) in a manner analogous to ubiquitylation (see UBE2M; MIM 603173) (Komatsu et al., 2004 [PubMed 15071506]).[supplied by OMIM, Dec 2008]

Uniprot Description

UFM1: Ubiquitin-like modifier protein which binds to a number of target proteins, such as DDRGK1. Belongs to the UFM1 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Ubiquitin-like modifier

Chromosomal Location of Human Ortholog: 13q13.3

Cellular Component: cytoplasm; endoplasmic reticulum; nucleus

Molecular Function: protein binding

Biological Process: negative regulation of apoptosis; regulation of intracellular estrogen receptor signaling pathway

Research Articles on UFM1

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Product Notes

The Pigeon UFM1 ufm1 (Catalog #AAA282698) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA282698 ELISA Kit recognizes Pigeon UFM1. It is sometimes possible for the material contained within the vial of "Ubiquitin-fold modifier 1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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