Human Potassium voltage-gated channel subfamily E member 1-like protein ELISA Kit | KCNE1L elisa kit

Human Potassium voltage-gated channel subfamily E member 1-like protein (KCNE1L) ELISA Kit

Cat. Num. AAA282519

• Gene Names: KCNE5; KCNE1L
• Reactivity: Human
• Synonyms: Potassium voltage-gated channel subfamily E member 1-like protein; Human Potassium voltage-gated channel subfamily E member 1-like protein (KCNE1L) ELISA Kit; KCNE1L elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Human KCNE1L. No significant cross-reactivity or interference between Human KCNE1L and analogues was observed.
• Sequence Length: 142

• Samples: Serum, Plasma, Other biological fluids
• Assay Type: Sandwich
• Sample Volume: 1-200 uL
• Precision: Intra-assay Precision (Precision within an assay); Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.; Inter-assay Precision (Precision between assays); Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.; CV (%) = SD/meanX100; Intra-Assay: CV; Inter-Assay: CV
• Detection Wavelength: 450 nm
• Preparation and Storage: Store at 2-8 degree C.

Related Product Information for KCNE1L elisa kit

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate KCNE1L in samples. An antibody specific for KCNE1L has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyKCNE1L present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for KCNE1L is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of KCNE1L bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 6912442
• NCBI GeneID: 23630
• NCBI Accession #: NP_036414.1
• NCBI GenBank Nucleotide #: NM_012282.3
• UniProt Accession #: Q9UJ90; Q5JWV7
• Molecular Weight: 14,993 Da
• NCBI Official Full Name: potassium voltage-gated channel subfamily E regulatory beta subunit 5
• NCBI Official Synonym Full Names: potassium voltage-gated channel subfamily E regulatory subunit 5
• NCBI Official Symbol: KCNE5
• NCBI Official Synonym Symbols: KCNE1L
• NCBI Protein Information: potassium voltage-gated channel subfamily E regulatory beta subunit 5
• UniProt Protein Name: Potassium voltage-gated channel subfamily E regulatory beta subunit 5
• UniProt Gene Name: KCNE5

NCBI Description

This gene encodes a member of a family of single pass transmembrane domain proteins that function as ancillary subunits to voltage-gated potassium channels. Members of this family affect diverse processes in potassium channel regulation, including ion selectivity, voltage dependence, and anterograde recycling from the plasma membrane. Variants of this gene are associated with idiopathic ventricular fibrillation and Brugada syndrome. [provided by RefSeq, Nov 2016]

Uniprot Description

KCNE1L: Defects in KCNE1L are involved in Alport syndrome with mental retardation midface hypoplasia and elliptocytosis (ATS-MR). A X-linked contiguous gene deletion syndrome characterized by glomerulonephritis, deafness, mental retardation, midface hypoplasia and elliptocytosis. Belongs to the potassium channel KCNE family.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: Xq23

Cellular Component: plasma membrane; voltage-gated potassium channel complex

Molecular Function: potassium channel regulator activity; protein binding; voltage-gated potassium channel activity

Biological Process: cardiac muscle contraction; regulation of heart contraction