Mouse X-ray radiation resistance-associated protein 1 ELISA Kit | XRRA1 elisa kit
Mouse X-ray radiation resistance-associated protein 1 (XRRA1) ELISA Kit
Gene Names
Xrra1; AI449753
Reactivity
Mouse
Synonyms
X-ray radiation resistance-associated protein 1; Mouse X-ray radiation resistance-associated protein 1 (XRRA1) ELISA Kit; XRRA1 elisa kit
Reactivity
Mouse
Specificity
This assay has high sensitivity and excellent specificity for detection of Mouse XRRA1. No significant cross-reactivity or interference between Mouse XRRA1 and analogues was observed.
Sequence Length
598
Samples
Serum, Plasma, Other biological fluids
Assay Type
Sandwich
Sample Volume
1-200 uL
Precision
Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
Detection Wavelength
450 nm
Preparation and Storage
Store at 2-8 degree C.
Related Product Information for XRRA1 elisa kit
The human XRRA1 gene encodes a deduced 559-amino acid protein with 2 possible sites of leucine-rich repeats, a PEST sequence, 2 tyrosine phosphorylation sites, and bipartite nuclear targeting sequences. XRRA1 gene appears to be highly conserved among mammals. RT-PCR detected the human XRRA1 transcript predominantly in testis, prostate, and ovary, and at variable levels in all cancer cell lines tested. DNA microarray analysis revealed a 3-fold greater expression of XRRA1 in macaque testis compared to mixed tissues, and similar levels of expression in macaque and human testis. Overexpression of a GFP-XRRA1 construct in HCT116 cells and COS-7 cells resulted in only transient expression, suggesting that levels of XRRA1 above the basal level may be lethal to cells. XRRA1 localized to the nucleus and cytoplasm of transfected cells.
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate XRRA1 in samples. An antibody specific for XRRA1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyXRRA1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for XRRA1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of XRRA1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate XRRA1 in samples. An antibody specific for XRRA1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyXRRA1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for XRRA1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of XRRA1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
88,884 Da
NCBI Official Full Name
X-ray radiation resistance-associated protein 1
NCBI Official Synonym Full Names
X-ray radiation resistance associated 1
NCBI Official Symbol
Xrra1
NCBI Official Synonym Symbols
AI449753
NCBI Protein Information
X-ray radiation resistance-associated protein 1
UniProt Protein Name
X-ray radiation resistance-associated protein 1
Protein Family
UniProt Gene Name
Xrra1
Uniprot Description
XRRA1: May be involved in the response of cells to X-ray radiation. 4 isoforms of the human protein are produced by alternative splicing.
Protein type: Unknown function
Chromosomal Location of Human Ortholog: 7|7 E2
Cellular Component: cytoplasm; nuclear body; nucleoplasm; nucleus
Biological Process: response to X-ray
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Product Notes
The Mouse XRRA1 xrra1 (Catalog #AAA282420) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA282420 ELISA Kit recognizes Mouse XRRA1. It is sometimes possible for the material contained within the vial of "X-ray radiation resistance-associated protein 1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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