Mouse Apolipoprotein B48 ELISA Kit | APO-B48 elisa kit

Mouse Apolipoprotein B48 ELISA Kit

Cat. Num. AAA28193

• Gene Names: APOB; FLDB; LDLCQ4
• Reactivity: Mouse
• Synonyms: Apolipoprotein B48; Mouse Apolipoprotein B48 ELISA Kit; APO-B48 elisa kit

• Reactivity: Mouse
• Sequence Length: 4563

• Assay Type: Quantitative Sandwich
• Detection Range: 3.12ug/ml-100ug/ml
• Sensitivity: 1.0ug/ml
• Intra-assay Precision: Intra-assay CV (%) is less than 15%.
• Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]. All CV% should be compared by concentration, not compared by OD values.
• Preparation and Storage: Store all reagents at 2-8 degree C

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for APO-B48 elisa kit

Background/Introduction: This Quantitative Sandwich ELISA kit is for lab reagent/research use only, not for drug, household, therapeutic or test applications! This kit is intended to be used for determine the level of APOB48 (hereafter termed "analyte") in undiluted original Mouse serum, plasma or tissue homogenates samples. For other sample types please contact tech support to determine compatibility with this assay. This kit is not suitable for assaying non-biological sources of substances.

NCBI and Uniprot Product Information

• NCBI GI #: 105990532
• NCBI GeneID: 338
• NCBI Accession #: NP_000375.2
• NCBI GenBank Nucleotide #: NM_000384.2
• UniProt Accession #: P04114; O00502; P78479; P78480; P78481; Q13779; Q13785; Q13786; Q13787; Q13788; Q4ZG63; Q53QC8
• Molecular Weight: 515,605 Da
• NCBI Official Full Name: apolipoprotein B-100
• NCBI Official Synonym Full Names: apolipoprotein B
• NCBI Official Symbol: APOB
• NCBI Official Synonym Symbols: FLDB; LDLCQ4
• NCBI Protein Information: apolipoprotein B-100; apoB-48; apoB-100; apo B-100; mutant Apo B 100; apolipoprotein B48; apolipoprotein B (including Ag(x) antigen)
• UniProt Protein Name: Apolipoprotein B-100
• UniProt Gene Name: APOB
• UniProt Synonym Gene Names: Apo B-100; Apo B-48
• UniProt Entry Name: APOB_HUMAN

NCBI Description

This gene product is the main apolipoprotein of chylomicrons and low density lipoproteins. It occurs in plasma as two main isoforms, apoB-48 and apoB-100: the former is synthesized exclusively in the gut and the latter in the liver. The intestinal and the hepatic forms of apoB are encoded by a single gene from a single, very long mRNA. The two isoforms share a common N-terminal sequence. The shorter apoB-48 protein is produced after RNA editing of the apoB-100 transcript at residue 2180 (CAA->UAA), resulting in the creation of a stop codon, and early translation termination. Mutations in this gene or its regulatory region cause hypobetalipoproteinemia, normotriglyceridemic hypobetalipoproteinemia, and hypercholesterolemia due to ligand-defective apoB, diseases affecting plasma cholesterol and apoB levels. [provided by RefSeq, Jul 2008]

Uniprot Description

APOB: Apolipoprotein B is a major protein constituent of chylomicrons (apo B-48), LDL (apo B-100) and VLDL (apo B-100). Apo B-100 functions as a recognition signal for the cellular binding and internalization of LDL particles by the apoB/E receptor. Defects in APOB are a cause of familial hypobetalipoproteinemia type 1 (FHBL1). A disorder characterized by highly reduced plasma concentrations of low density lipoproteins, and dietary fat malabsorption. Clinical presentation may vary from no symptoms to severe gastrointestinal and neurological dysfunction similar to abetalipoproteinemia. Defects in APOB are a cause of familial ligand-defective apolipoprotein B-100 (FDB). FDB is a dominantly inherited disorder of lipoprotein metabolism leading to hypercholesterolemia and increased proneness to coronary artery disease (CAD). The plasma cholesterol levels are dramatically elevated due to impaired clearance of LDL particles by defective APOB/E receptors. Defects in APOB associated with defects in other genes (polygenic) can contribute to hypocholesterolemia.

Protein type: Carrier; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 2p24-p23

Cellular Component: Golgi apparatus; extracellular space; endoplasmic reticulum membrane; chylomicron; intracellular membrane-bound organelle; cell soma; endoplasmic reticulum lumen; cytoplasm; early endosome; extracellular region; plasma membrane; endosome membrane; cytosol; actin cytoskeleton

Molecular Function: heparin binding; protein binding; low-density lipoprotein receptor binding; cholesterol transporter activity; phospholipid binding

Biological Process: phototransduction, visible light; lipoprotein catabolic process; response to lipopolysaccharide; response to carbohydrate stimulus; post-embryonic development; response to selenium ion; triacylglycerol catabolic process; retinoid metabolic process; transmembrane transport; cholesterol metabolic process; nervous system development; receptor-mediated endocytosis; cholesterol transport; in utero embryonic development; response to virus; regulation of cholesterol biosynthetic process; lipoprotein metabolic process; cholesterol efflux; sperm motility; cholesterol homeostasis; fertilization; lipoprotein transport; lipoprotein biosynthetic process; artery morphogenesis; spermatogenesis; triacylglycerol mobilization; blood coagulation; leukocyte migration

Disease: Hypercholesterolemia, Autosomal Dominant, Type B; Hypobetalipoproteinemia, Familial, 1