Horse Thyrotropin subunit beta ELISA Kit | TSHB elisa kit
Horse Thyrotropin subunit beta (TSHB) ELISA Kit
Gene Names
TSHB; TSH-BETA
Reactivity
Horse
Synonyms
Thyrotropin subunit beta; Horse Thyrotropin subunit beta (TSHB) ELISA Kit; TSHB elisa kit
Reactivity
Horse
Specificity
This assay has high sensitivity and excellent specificity for detection of Horse TSHB. No significant cross-reactivity or interference between Horse TSHB and analogues was observed.
Sequence Length
138
Samples
serum, plasma and other biological fluids
Assay Type
Quantitative Sandwich
Detection Range
0.31 ng/mL - 20 ng/mL.
Intra-assay Precision
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Intra-Assay: CV<8%
Inter-assay Precision
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in forty separate assays to assess inter-assay precision. CV (%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
Store at 2-8 degree C.
Related Product Information for TSHB elisa kit
Intended Uses: For the quantitative detection of Horse Thyrotropin subunit beta (TSHB) concentration in serum, plasma and other biological fluids.
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate TSHB in Horse serum, plasma. An antibody specific for TSHB has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TSHB present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TSHB is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TSHB bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Thyrotropin-stimulating hormone (TSH) is a noncovalently linked glycoprotein heterodimer and is part of a family of pituitary hormones containing a common alpha subunit (TSHA) and a unique beta subunit that confers specificity.Using bovine TSHB cDNA as probe, Hayashizaki et al. (1985) cloned TSHB from human liver and leukocyte genomic DNA libraries. Human TSHB encodes a deduced protein consisting of a 20-amino acid signal sequence, a mature protein of 112 amino acids, and a C-terminal extension of 6 hydrophobic amino acids. Wondisford et al. (1988) also cloned the human TSHB gene.Wondisford et al. (1988) determined that the TSHB gene contains 3 exons, the first of which is noncoding. The rat TSHB gene also contains one 5-prime noncoding exon, whereas the mouse Tshb gene contains 3.
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate TSHB in Horse serum, plasma. An antibody specific for TSHB has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TSHB present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TSHB is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TSHB bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Thyrotropin-stimulating hormone (TSH) is a noncovalently linked glycoprotein heterodimer and is part of a family of pituitary hormones containing a common alpha subunit (TSHA) and a unique beta subunit that confers specificity.Using bovine TSHB cDNA as probe, Hayashizaki et al. (1985) cloned TSHB from human liver and leukocyte genomic DNA libraries. Human TSHB encodes a deduced protein consisting of a 20-amino acid signal sequence, a mature protein of 112 amino acids, and a C-terminal extension of 6 hydrophobic amino acids. Wondisford et al. (1988) also cloned the human TSHB gene.Wondisford et al. (1988) determined that the TSHB gene contains 3 exons, the first of which is noncoding. The rat TSHB gene also contains one 5-prime noncoding exon, whereas the mouse Tshb gene contains 3.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
15,663 Da
NCBI Official Full Name
thyrotropin subunit beta
NCBI Official Synonym Full Names
thyroid stimulating hormone beta
NCBI Official Symbol
TSHB
NCBI Official Synonym Symbols
TSH-BETA
NCBI Protein Information
thyrotropin subunit beta
UniProt Protein Name
Thyrotropin subunit beta
Protein Family
UniProt Gene Name
TSHB
UniProt Synonym Gene Names
TSH-B; TSH-beta
Uniprot Description
Indispensable for the control of thyroid structure and metabolism.
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Product Notes
The Horse TSHB tshb (Catalog #AAA281389) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA281389 ELISA Kit recognizes Horse TSHB. It is sometimes possible for the material contained within the vial of "Thyrotropin subunit beta, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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