Human Plasma kallikrein ELISA Kit | KLKB1 elisa kit

Human Plasma kallikrein (KLKB1) ELISA Kit

Cat. Num. AAA281175

• Gene Names: Klkb1; APS; PSA; Kal3; Klk3; Kal-3
• Reactivity: Human
• Synonyms: Plasma kallikrein; Human Plasma kallikrein (KLKB1) ELISA Kit; KLKB1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Human KLKB1. No significant cross-reactivity or interference between Human KLKB1 and analogues was observed.
• Sequence Length: 638

• Samples: Serum, Plasma, Other biological fluids
• Assay Type: Sandwich
• Sample Volume: 1-200 uL
• Precision: Intra-assay Precision (Precision within an assay); Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.; Inter-assay Precision (Precision between assays); Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.; CV (%) = SD/meanX100; Intra-Assay: CV; Inter-Assay: CV
• Detection Wavelength: 450 nm
• Preparation and Storage: Store at 2-8 degree C.

Related Product Information for KLKB1 elisa kit

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate KLKB1 in samples. An antibody specific for KLKB1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyKLKB1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for KLKB1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of KLKB1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 236465805
• NCBI GeneID: 16621
• NCBI Accession #: NP_032481.2
• NCBI GenBank Nucleotide #: NM_008455.2
• Molecular Weight: 71,383 Da
• NCBI Official Full Name: plasma kallikrein
• NCBI Official Synonym Full Names: kallikrein B, plasma 1
• NCBI Official Symbol: Klkb1
• NCBI Official Synonym Symbols: APS; PSA; Kal3; Klk3; Kal-3
• NCBI Protein Information: plasma kallikrein; kininogenin; fletcher factor; kallikrein 3, plasma; plasma prekallikrein; antigen, prostate specific
• UniProt Protein Name: Plasma kallikrein
• Protein Family: Plasma kallikrein
• UniProt Gene Name: Klkb1
• UniProt Synonym Gene Names: Klk3; Pk
• UniProt Entry Name: KLKB1_MOUSE

Uniprot Description

KLKB1: The enzyme cleaves Lys-Arg and Arg-Ser bonds. It activates, in a reciprocal reaction, factor XII after its binding to a negatively charged surface. It also releases bradykinin from HMW kininogen and may also play a role in the renin-angiotensin system by converting prorenin into renin. Defects in KLKB1 are the cause of prekallikrein deficiency (PKK deficiency); also known as Fletcher factor deficiency. This disorder is a blood coagulation defect. Belongs to the peptidase S1 family. Plasma kallikrein subfamily.

Protein type: EC 3.4.21.34; Protease; Secreted; Secreted, signal peptide

Cellular Component: extracellular space; extracellular region

Molecular Function: peptidase activity; hydrolase activity; serine-type peptidase activity; serine-type endopeptidase activity; catalytic activity

Biological Process: fibrinolysis; hemostasis; positive regulation of fibrinolysis; proteolysis; inflammatory response; blood coagulation; plasminogen activation