Hamster Thyroxine-Binding Globulin ELISA Kit | TBG elisa kit

Hamster Thyroxine-Binding Globulin (TBG) ELISA Kit

Cat. Num. AAA281144

• Gene Names: SERPINA7; TBG
• Reactivity: Hamster
• Synonyms: Thyroxine-Binding Globulin; Hamster Thyroxine-Binding Globulin (TBG) ELISA Kit; TBG elisa kit

• Reactivity: Hamster
• Specificity: This assay has high sensitivity and excellent specificity for detection of Hamster (Mesocricetus; Cricetulus)TBG. No significant cross-reactivity or interference between Hamster (Mesocricetus; Cricetulus)TBG and analogues was observed.
• Sequence Length: 415

• Samples: Serum, Plasma, Other biological fluids
• Assay Type: Sandwich
• Sample Volume: 1-200 uL
• Precision: Intra-assay Precision (Precision within an assay); Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.; Inter-assay Precision (Precision between assays); Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.; CV (%) = SD/meanX100; Intra-Assay: CV; Inter-Assay: CV
• Detection Wavelength: 450 nm
• Preparation and Storage: Store at 2-8 degree C.

Related Product Information for TBG elisa kit

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate TBG in samples. An antibody specific for TBG has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTBG present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TBG is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TBG bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 205277441
• NCBI GeneID: 6906
• NCBI Accession #: NP_000345.2
• NCBI GenBank Nucleotide #: NM_000354.5
• Molecular Weight: 46,325 Da
• NCBI Official Full Name: thyroxine-binding globulin
• NCBI Official Synonym Full Names: serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 7
• NCBI Official Symbol: SERPINA7
• NCBI Official Synonym Symbols: TBG
• NCBI Protein Information: thyroxine-binding globulin; serpin A7; T4-binding globulin; thyroxin-binding globulin; serine (or cysteine) proteinase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 7
• UniProt Protein Name: Thyroxine-binding globulin
• Protein Family: Thyroxine-binding globulin
• UniProt Gene Name: SERPINA7
• UniProt Synonym Gene Names: TBG
• UniProt Entry Name: THBG_HUMAN

NCBI Description

There are three proteins including thyroxine-binding globulin (TBG), transthyretin and albumin responsible for carrying the thyroid hormones thyroxine (T4) and 3,5,3'-triiodothyronine (T3) in the bloodstream. This gene encodes the major thyroid hormone transport protein, TBG, in serum. It belongs to the serpin family in genomics, but the protein has no inhibitory function like many other members of the serpin family. Mutations in this gene result in TGB deficiency, which has been classified as partial deficiency, complete deficiency, and excess, based on the level of serum TBG. Alternatively spliced transcript variants encoding different isoforms have been found, but the full-length nature of these variants has not been determined.[provided by RefSeq, Jun 2012]

Uniprot Description

SERPINA7: Major thyroid hormone transport protein in serum. Defects in SERPINA7 are a cause of thyroxine-binding globulin deficiency (TBG deficiency). Mutations in the SERPINA7 gene can result as a whole spectrum of deficiencies, characterized by either reduced or increased TBG levels in the serum. Patients show, respectively, reduced or elevated protein- bound iodine but are euthyroid. Belongs to the serpin family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: Xq22.2

Cellular Component: extracellular space; extracellular region

Molecular Function: serine-type endopeptidase inhibitor activity; hormone binding

Biological Process: response to drug; response to peptide hormone stimulus; aging; post-embryonic development; response to vitamin A; response to corticosterone stimulus