Human Protein Wnt-2b ELISA Kit | WNT2B elisa kit

Human Protein Wnt-2b (WNT2B) ELISA Kit

Cat. Num. AAA281073

• Gene Names: WNT2B; WNT13
• Reactivity: Human
• Synonyms: Protein Wnt-2b; Human Protein Wnt-2b (WNT2B) ELISA Kit; WNT2B elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Human WNT2B. No significant cross-reactivity or interference between Human WNT2B and analogues was observed.
• Sequence Length: 299

• Samples: Serum, Plasma, Other biological fluids
• Assay Type: Sandwich
• Sample Volume: 1-200 uL
• Precision: Intra-assay Precision (Precision within an assay); Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.; Inter-assay Precision (Precision between assays); Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.; CV (%) = SD/meanX100; Intra-Assay: CV; Inter-Assay: CV
• Detection Wavelength: 450 nm
• Preparation and Storage: Store at 2-8 degree C.

Related Product Information for WNT2B elisa kit

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate WNT2B in samples. An antibody specific for WNT2B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyWNT2B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for WNT2B is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of WNT2B bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 630044901
• NCBI GeneID: 7482
• NCBI Accession #: NP_001278809.1
• NCBI GenBank Nucleotide #: NM_001291880.1
• UniProt Accession #: Q93097; O14903; Q5TEH9; Q5TEI2; Q9HDC1; Q9HDC2
• Molecular Weight: 41,830 Da
• NCBI Official Full Name: protein Wnt-2b isoform 3
• NCBI Official Synonym Full Names: Wnt family member 2B
• NCBI Official Symbol: WNT2B
• NCBI Official Synonym Symbols: WNT13
• NCBI Protein Information: protein Wnt-2b
• UniProt Protein Name: Protein Wnt-2b
• Protein Family: Protein
• UniProt Gene Name: WNT2B
• UniProt Synonym Gene Names: WNT13

NCBI Description

This gene encodes a member of the wingless-type MMTV integration site (WNT) family of highly conserved, secreted signaling factors. WNT family members function in a variety of developmental processes including regulation of cell growth and differentiation and are characterized by a WNT-core domain. This gene may play a role in human development as well as carcinogenesis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2014]

Uniprot Description

WNT2B: Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters. May be involved in normal development or differentiation as well as in carcinogenesis. Belongs to the Wnt family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 1p13.2

Cellular Component: extracellular region; extracellular space; intracellular membrane-bound organelle; proteinaceous extracellular matrix

Molecular Function: frizzled binding

Biological Process: cell fate commitment; cellular response to starvation; chondrocyte differentiation; forebrain regionalization; lens development in camera-type eye; male gonad development; neuron differentiation; Wnt receptor signaling pathway through beta-catenin; Wnt signaling pathway