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Human Glucose dependent insulin releasing polypeptide ELISA Kit | GIP elisa kit

Human Glucose dependent insulin releasing polypeptide (GIP) ELISA Kit

Reactivity
Human
Synonyms
Glucose dependent insulin releasing polypeptide; Human Glucose dependent insulin releasing polypeptide (GIP) ELISA Kit; GIP elisa kit
Ordering
For Research Use Only!
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of Human GIP. No significant cross-reactivity or interference between Human GIP and analogues was observed.
Samples
Serum, Plasma, Other biological fluids
Assay Type
Sandwich
Sample Volume
1-200 uL
Precision
Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
Detection Wavelength
450 nm
Preparation and Storage
Store at 2-8 degree C.
Related Product Information for GIP elisa kit
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate GIP in samples. An antibody specific for GIP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyGIP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GIP is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GIP bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
17,108 Da
NCBI Official Full Name
gastric inhibitory polypeptide preproprotein
NCBI Official Synonym Full Names
gastric inhibitory polypeptide
NCBI Official Symbol
GIP
NCBI Protein Information
gastric inhibitory polypeptide
UniProt Protein Name
Gastric inhibitory polypeptide
UniProt Gene Name
GIP
UniProt Synonym Gene Names
GIP

NCBI Description

This gene encodes an incretin hormone and belongs to the glucagon superfamily. The encoded protein is important in maintaining glucose homeostasis as it is a potent stimulator of insulin secretion from pancreatic beta-cells following food ingestion and nutrient absorption. This gene stimulates insulin secretion via its G protein-coupled receptor activation of adenylyl cyclase and other signal transduction pathways. It is a relatively poor inhibitor of gastric acid secretion. [provided by RefSeq, Jul 2008]

Uniprot Description

GIP: Potent stimulator of insulin secretion and relatively poor inhibitor of gastric acid secretion. Belongs to the glucagon family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 17q21.32

Cellular Component: cell soma; endoplasmic reticulum lumen; extracellular region; extracellular space

Molecular Function: hormone activity; protein binding

Biological Process: adult locomotory behavior; endocrine pancreas development; female pregnancy; memory; positive regulation of insulin secretion; regulation of insulin secretion; response to acidic pH; response to amino acid; response to axon injury; response to drug; response to glucose stimulus; response to lipid; response to organic cyclic compound; response to peptide hormone; response to selenium ion; response to starvation; sensory perception of pain; signal transduction

Research Articles on GIP

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Product Notes

The Human GIP gip (Catalog #AAA281017) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA281017 ELISA Kit recognizes Human GIP. It is sometimes possible for the material contained within the vial of "Glucose dependent insulin releasing polypeptide, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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