Sheep T-cell surface glycoprotein CD3 delta chain ELISA Kit | CD3D elisa kit

Sheep T-cell surface glycoprotein CD3 delta chain (CD3D) ELISA Kit

Cat. Num. AAA280914

• Reactivity: Sheep
• Synonyms: T-cell surface glycoprotein CD3 delta chain; Sheep T-cell surface glycoprotein CD3 delta chain (CD3D) ELISA Kit; CD3D elisa kit

• Reactivity: Sheep
• Specificity: This assay has high sensitivity and excellent specificity for detection of Sheep CD3D. No significant cross-reactivity or interference between Sheep CD3D and analogues was observed.
• Sequence Length: 171

• Samples: Serum, Plasma, Other biological fluids
• Assay Type: Sandwich
• Sample Volume: 1-200 uL
• Precision: Intra-assay Precision (Precision within an assay); Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.; Inter-assay Precision (Precision between assays); Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.; CV (%) = SD/meanX100; Intra-Assay: CV; Inter-Assay: CV
• Detection Wavelength: 450 nm
• Preparation and Storage: Store at 2-8 degree C.

Related Product Information for CD3D elisa kit

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate CD3D in samples. An antibody specific for CD3D has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyCD3D present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CD3D is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CD3D bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 57164385
• NCBI GeneID: 443397
• NCBI Accession #: NP_001009382.1
• NCBI GenBank Nucleotide #: NM_001009382.1
• UniProt Accession #: P18438
• Molecular Weight: 18,562 Da
• NCBI Official Full Name: T-cell surface glycoprotein CD3 delta chain
• NCBI Official Symbol: CD3D
• NCBI Protein Information: T-cell surface glycoprotein CD3 delta chain
• UniProt Protein Name: T-cell surface glycoprotein CD3 delta chain
• Protein Family: T-cell surface glycoprotein
• UniProt Gene Name: CD3D

Uniprot Description

Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways. In addition of this role of signal transduction in T-cell activation, CD3D plays an essential role in thymocyte differentiation. Indeed, participates in correct intracellular TCR-CD3 complex assembly and surface expression. In absence of a functional TCR-CD3 complex, thymocytes are unable to differentiate properly. Interacts with CD4 and CD8 and thus serves to establish a functional link between the TCR and coreceptors CD4 and CD8, which is needed for activation and positive selection of CD4 or CD8 T-cells.