Rat Acyl-coenzyme A thioesterase 2 ELISA Kit | ACOT2 elisa kit
Rat Acyl-coenzyme A thioesterase 2, mitochondrial (ACOT2) ELISA Kit
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate ACH in Rat serum, plasma. An antibody specific for ACH has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ACH present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ACH is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ACH bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI and Uniprot Product Information
NCBI Description
hydrolase that is inducible by peroxisome proliferators; may have a role in lipid metabolism [RGD, Feb 2006]
Uniprot Description
ACOT2: Acyl-CoA thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH. Displays high levels of activity on medium- and long chain acyl CoAs. Belongs to the C/M/P thioester hydrolase family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: EC 3.1.2.2; Hydrolase; Lipid Metabolism - unsaturated fatty acid biosynthesis; Mitochondrial
Chromosomal Location of Human Ortholog: 6q24
Cellular Component: mitochondrial matrix; mitochondrion
Molecular Function: acyl-CoA hydrolase activity; palmitoyl-CoA hydrolase activity; receptor binding; thiolester hydrolase activity
Biological Process: acyl-CoA metabolic process; long-chain fatty acid metabolic process; response to drug; response to hormone; response to hypoxia; response to nutrient levels; very long-chain fatty acid metabolic process; very-long-chain fatty acid catabolic process
Research Articles on ACOT2
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Product Notes
The Rat ACOT2 acot2 (Catalog #AAA280473) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA280473 ELISA Kit recognizes Rat ACOT2. It is sometimes possible for the material contained within the vial of "Acyl-coenzyme A thioesterase 2, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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