Human Pleckstrin homology domain-containing family F member 1 ELISA Kit | PLEKHF1 elisa kit
Human Pleckstrin homology domain-containing family F member 1 (PLEKHF1) ELISA Kit
Gene Names
PLEKHF1; APPD; LAPF; PHAFIN1; ZFYVE15
Reactivity
Human
Synonyms
Pleckstrin homology domain-containing family F member 1; Human Pleckstrin homology domain-containing family F member 1 (PLEKHF1) ELISA Kit; PLEKHF1 elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of Human PHOSPHO1. No significant cross-reactivity or interference between Human PHOSPHO1 and analogues was observed.
Sequence Length
279
Samples
Serum, Plasma And Other Biological Fluids
Assay Type
Sandwich
Detection Range
12.5 pg/mL-800 pg/mL
Sensitivity
6.25 pg/mL (mean of 6 independent assays).
Intra-assay Precision
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Intra-Assay: CV<8%
Inter-assay Precision
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in forty separate assays to assess inter-assay precision. CV (%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
Store at 2-8 degree C.
Related Product Information for PLEKHF1 elisa kit
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate PHOSPHO1 in Human serum, plasma. An antibody specific for PHOSPHO1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PHOSPHO1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PHOSPHO1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PHOSPHO1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
31,195 Da
NCBI Official Full Name
pleckstrin homology domain-containing family F member 1
NCBI Official Synonym Full Names
pleckstrin homology and FYVE domain containing 1
NCBI Official Symbol
PLEKHF1
NCBI Official Synonym Symbols
APPD; LAPF; PHAFIN1; ZFYVE15
NCBI Protein Information
pleckstrin homology domain-containing family F member 1
UniProt Protein Name
Pleckstrin homology domain-containing family F member 1
Protein Family
UniProt Gene Name
PLEKHF1
UniProt Synonym Gene Names
APPD; LAPF; ZFYVE15; PH domain-containing family F member 1; Apoptosis-inducing protein
Uniprot Description
PKHF1: May induce apoptosis through the lysosomal-mitochondrial pathway. Translocates to the lysosome initiating the permeabilization of lysosomal membrane (LMP) and resulting in the release of CTSD and CTSL to the cytoplasm. Triggers the caspase- independent apoptosis by altering mitochondrial membrane permeabilization (MMP) resulting in the release of PDCD8.
Chromosomal Location of Human Ortholog: 19q12
Cellular Component: endosome; endosome membrane; lysosomal membrane; lysosome; nucleus; perinuclear region of cytoplasm
Molecular Function: metal ion binding; phosphatidylinositol 3-phosphate binding; phosphatidylinositol-5-phosphate binding; protein binding
Biological Process: apoptosis; endosome organization and biogenesis; positive regulation of autophagy; vesicle organization and biogenesis
Research Articles on PLEKHF1
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Product Notes
The Human PLEKHF1 plekhf1 (Catalog #AAA280383) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA280383 ELISA Kit recognizes Human PLEKHF1. It is sometimes possible for the material contained within the vial of "Pleckstrin homology domain-containing family F member 1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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