Human Protein-arginine deiminase type-6 ELISA Kit | PADI6 elisa kit

Human Protein-arginine deiminase type-6 (PADI6) ELISA Kit

Cat. Num. AAA280343

• Gene Names: PADI6; hPADVI; PREMBL2
• Reactivity: Human
• Synonyms: Protein-arginine deiminase type-6; Human Protein-arginine deiminase type-6 (PADI6) ELISA Kit; PADI6 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Human PADI6. No significant cross-reactivity or interference between Human PADI6 and analogues was observed.
• Sequence Length: 694

• Samples: Serum, Plasma, Other biological fluids
• Assay Type: Sandwich
• Sample Volume: 1-200 uL
• Precision: Intra-assay Precision (Precision within an assay); Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.; Inter-assay Precision (Precision between assays); Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.; CV (%) = SD/meanX100; Intra-Assay: CV; Inter-Assay: CV
• Detection Wavelength: 450 nm
• Preparation and Storage: Store at 2-8 degree C.

Related Product Information for PADI6 elisa kit

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate PADI6 in samples. An antibody specific for PADI6 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPADI6 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PADI6 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PADI6 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 117414158
• NCBI GeneID: 353238
• NCBI Accession #: NP_997304.3
• NCBI GenBank Nucleotide #: NM_207421.4
• UniProt Accession #: Q6TGC4; Q330K5; Q70SX3
• Molecular Weight: 77,727 Da
• NCBI Official Full Name: protein-arginine deiminase type-6
• NCBI Official Synonym Full Names: peptidyl arginine deiminase 6
• NCBI Official Symbol: PADI6
• NCBI Official Synonym Symbols: hPADVI; PREMBL2
• NCBI Protein Information: protein-arginine deiminase type-6
• UniProt Protein Name: Protein-arginine deiminase type-6
• Protein Family: Protein-arginine deiminase
• UniProt Gene Name: PADI6
• UniProt Synonym Gene Names: PAD6; hPADVI

NCBI Description

This gene encodes a member of the peptidyl arginine deiminase family of enzymes, which catalyze the post-translational deimination of proteins by converting arginine residues into citrullines in the presence of calcium ions. The family members have distinct substrate specificities and tissue-specific expression patterns. This protein may play a role in cytoskeletal reorganization in the egg and in early embryo development. [provided by RefSeq, Sep 2012]

Uniprot Description

PADI6: Catalyzes the deimination of arginine residues of proteins. May be involved in cytoskeletal reorganization in the egg and early embryo. Belongs to the protein arginine deiminase family.

Protein type: Cytoskeletal; EC 3.5.3.15; Hydrolase

Chromosomal Location of Human Ortholog: 1p36.13

Cellular Component: cytoplasm; intermediate filament cytoskeleton; nucleus

Molecular Function: calcium ion binding; protein-arginine deiminase activity

Biological Process: cytoplasm organization and biogenesis; cytoskeleton organization; regulation of translation by machinery localization

Disease: Preimplantation Embryonic Lethality 2