Mouse Zinc finger E-box-binding homeobox 1 ELISA Kit | ZEB1 elisa kit
Mouse Zinc finger E-box-binding homeobox 1 (ZEB1) ELISA Kit
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
Delta-Ef1 selectively transactivated the promoters of the SMC differentiation marker genes Acta2 and Myh11, and it physically interacted with Srf and Smad3, resulting in synergistic activation of the Acta2 promoter. Chromatin immunoprecipitation assays and knockdown experiments showed that delta-Ef1 was involved in control of SMC differentiation programs induced by Tgf-beta (TGFB1) signaling. Overexpression of delta-Ef1 inhibited neointima formation and promoted SMC differentiation.
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate ZEB1 in samples. An antibody specific for ZEB1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyZEB1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ZEB1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ZEB1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI and Uniprot Product Information
Uniprot Description
TCF8: Acts as a transcriptional repressor. Inhibits interleukin-2 (IL-2) gene expression. Enhances or represses the promoter activity of the ATP1A1 gene depending on the quantity of cDNA and on the cell type. Represses E-cadherin promoter and induces an epithelial-mesenchymal transition (EMT) by recruiting SMARCA4/BRG1. Represses BCL6 transcription in the presence of the corepressor CTBP1. Positively regulates neuronal differentiation. Represses RCOR1 transcription activation during neurogenesis. Represses transcription by binding to the E box (5'-CANNTG-3'). Promotes tumorigenicity by repressing stemness-inhibiting microRNAs. Interacts (via N-terminus) with SMARCA4/BRG1. Colocalizes with SMARCA4/BRG1 in E-cadherin- negative cells from established lines, and stroma of normal colon as well as in de-differentiated epithelial cells at the invasion front of colorectal carcinomas. Expressed in heart and skeletal muscle, but not in liver, spleen, or pancreas. Belongs to the delta-EF1/ZFH-1 C2H2-type zinc-finger family.
Protein type: C2H2-type zinc finger protein; DNA-binding; Motility/polarity/chemotaxis; Transcription, coactivator/corepressor
Chromosomal Location of Human Ortholog: 18 4.42 cM|18 A1
Cellular Component: cytosol; nucleoplasm; nucleus; transcription factor complex
Molecular Function: chromatin binding; DNA binding; DNA binding transcription factor activity; double-stranded DNA binding; metal ion binding; nucleic acid binding; protein binding; sequence-specific DNA binding; transcription factor binding
Biological Process: animal organ development; cartilage development; cell differentiation; central nervous system development; cochlea morphogenesis; embryonic camera-type eye morphogenesis; embryonic morphogenesis; embryonic skeletal morphogenesis; negative regulation of cell proliferation; negative regulation of endothelial cell differentiation; negative regulation of epithelial cell differentiation; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; nervous system development; pattern specification process; positive regulation of neuron differentiation; positive regulation of transcription from RNA polymerase II promoter; regulation of mesenchymal cell proliferation; regulation of smooth muscle cell differentiation; regulation of T cell differentiation in the thymus; regulation of transcription, DNA-templated; regulation of transforming growth factor beta receptor signaling pathway; semicircular canal morphogenesis; transcription, DNA-dependent
Research Articles on ZEB1
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Product Notes
The Mouse ZEB1 zeb1 (Catalog #AAA280255) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA280255 ELISA Kit recognizes Mouse ZEB1. It is sometimes possible for the material contained within the vial of "Zinc finger E-box-binding homeobox 1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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