Human Protein NLRC5 (NLRC5) ELISA Kit | NLRC5 elisa kit

Human Protein NLRC5 (NLRC5) ELISA Kit

Cat. Num. AAA2801706

• Gene Names: NLRC5; NOD4; NOD27; CLR16.1
• Reactivity: Human
• Synonyms: Protein NLRC5 (NLRC5); Human Protein NLRC5 (NLRC5) ELISA Kit; CLR16.1; FLJ21709; FLJ39711; NOD27; NOD4; NOD-like receptor C5; nucleotide-binding oligomerization domain; leucine rich repeat and CARD domain containing 5; nucleotide-binding oligomerization domains; NLRC5 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Human NLRC5. No significant cross-reactivity or interference between Human NLRC5 and analogues was observed.

• Asssay Type: Quantitive Sandwich
• Sample Types: Serum, Plasma, Other biological fluids
• Detection Wavelength: 450nm
• Sample Volume: 1-200uL
• Precision: Intra-assay Precision (Precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.; Inter-assay Precision (Precision between assays): Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.; CV (%) = SD/meanX100; Intra-Assay: CV<8%; Inter-Assay: CV<12%
• Test Principle: This assay employs a two-site sandwich ELISA to quantitate NLRC5 in samples. An antibody specific for NLRC5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any NLRC5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NLRC5 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NLRC5 bound in the initial step. The color development is stopped and the intensity of the color is measured.
• Assay Duration: 1-4.5h
• Preparation and Storage: Store at 2-8 degree C

NCBI and Uniprot Product Information

• NCBI GI #: 116268109
• NCBI GeneID: 84166
• NCBI Accession #: NP_115582
• NCBI GenBank Nucleotide #: NM_032206.3
• UniProt Accession #: Q86WI3; Q6P4A6; Q86VM7; Q8NF42; Q8TEE2; Q8TEJ1; Q969L7; B5MEF1; C9JMD8
• Molecular Weight: 205 kDa
• NCBI Official Full Name: protein NLRC5
• NCBI Official Synonym Full Names: NLR family, CARD domain containing 5
• NCBI Official Symbol: NLRC5
• NCBI Official Synonym Symbols: NOD4; NOD27; CLR16.1
• NCBI Protein Information: protein NLRC5; NOD-like receptor C5; caterpiller protein 16.1; nucleotide-binding oligomerization domains 27; nucleotide-binding oligomerization domain protein 4; nucleotide-binding oligomerization domain, leucine rich repeat and CARD domain containing 5
• UniProt Protein Name: Protein NLRC5
• Protein Family: Protein
• UniProt Gene Name: NLRC5
• UniProt Synonym Gene Names: NOD27; NOD4; CLR16.1
• UniProt Entry Name: NLRC5_HUMAN

NCBI Description

This gene encodes a member of the caspase recruitment domain-containing NLR family. This gene plays a role in cytokine response and antiviral immunity through its inhibition of NF-kappa-B activation and negative regulation of type I interferon signaling pathways. [provided by RefSeq, Oct 2011]

Uniprot Description

NLRC5: Probable regulator of the NF-kappa-B and type I interferon signaling pathways. May also regulate the type II interferon signaling pathway. Plays a role in homeostatic control of innate immunity and in antiviral defense mechanisms. Belongs to the NLRP family. 6 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 16q13

Cellular Component: cytoplasm; nucleus; cytosol

Molecular Function: protein binding; ATP binding

Biological Process: regulation of kinase activity; inhibition of NF-kappaB transcription factor; innate immune response; positive regulation of MHC class I biosynthetic process; positive regulation of transcription from RNA polymerase II promoter; defense response to virus; negative regulation of interferon type I production